Direct flowcytometric analysis of eosinophils using a whole blood staining technique

Allergology International

Volumn 50, Issue 4, 2001, Pages 319-324

  Kamada, Yumiko ^a   Tsuda, Akira ^a   Kayaba, Hiroyuki ^a   Urayama, Osamu ^a   Oyamada, Hajime ^a   Kuwasaki, Tomoe ^a   Takasaki, Kiumi ^a   Saito, Norihiro ^a   Chihara, Junichi ^a  

^a AKITA UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

EG2; Eosinophils; Flow cytometry; Whole blood

Indexed keywords

1,4 BENZOQUINONE; CD16 ANTIGEN; CD9 ANTIGEN; EDETIC ACID; EOSINOPHIL CATIONIC PROTEIN; OCTYL GLUCOSIDE; PARAFORMALDEHYDE;

ADULT; AGED; ARTICLE; BLOOD STAIN; CELL MEMBRANE PERMEABILITY; CLINICAL ARTICLE; CONTROLLED STUDY; EOSINOPHIL; FLOW CYTOMETRY; FLUORESCENCE; HUMAN; LEUKOCYTE; PRIORITY JOURNAL; TECHNIQUE;

EID: 0035205058     PISSN: 13238930     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1440-1592.2001.00233.x     Document Type: Article

References (14)

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3. The methods for eosinophilpurification
4. The FOG method: A new approach to detecting activated eosinophils
5. A flowcytometric method for the detection of intracellular basic proteins in unseparated peripheral blood and bone marrow eosinophils
6. Monoclonal antibodies distinguish between storage and secreted forms of eosinophil cationic protein
7. A new membrane permeabilization method for the detection of intracellular antigens by flow cytometry
8. Identification of eosinophils by flow cytometry
9. Detection of eosinophils in whole blood samples by flow cytometry
10. Change in expression of Fc gamma RIII (CD16) on neutrophils from human immunodeficiency virus-infected individuals
11. Whole blood flow, cytometric analysis of eosinophil EG2 expression as a marker of the pathological conditions of asthma
12. Eosinophil activity markers in peripheral blood have high predictive value for bronchial hyperreactivity in patients with suspected mild asthma
13. The degree of natural allergen exposure modifies eosinophil activity markers in the circulation of patients with mild asthma
14. CD44 expression on blood eosinophils is a novel marker of bronchial asthma