A moderate amplification of the mecB gene encoding cystathionine-γ-lyase stimulates cephalosporin biosynthesis in acremonium chrysogenum

Journal of Industrial Microbiology and Biotechnology

Volumn 27, Issue 4, 2001, Pages 252-258

  Kosalkova K ^a   Marcos, A T ^b   Martin J F ^a  

^a UNIVERSITY OF LEÓN   (Spain)

^b Institute of Biotechnology INBIOTEC Parque Científico de León   (Spain)

Author keywords

Cephalosporin biosynthesis; Cystathionine lyase; Gene amplification; MecB; Sulfur metabolism

Indexed keywords

CEPHALOSPORIN; CYSTATHIONINE GAMMA LYASE;

ARTICLE; CEPHALOSPORIUM ACREMONIUM; CONTROLLED STUDY; CULTURE MEDIUM; DRUG SYNTHESIS; ENZYME ACTIVITY; GENE AMPLIFICATION; GENE EXPRESSION; GENE ISOLATION; GENETIC CODE; GENETIC TRANSCRIPTION; MOLECULAR CLONING; NONHUMAN; NORTHERN BLOTTING; SOUTHERN BLOTTING; TITRIMETRY;

ACREMONIUM; BIOTECHNOLOGY; CEPHALOSPORINS; CULTURE MEDIA; CYSTATHIONINE GAMMA-LYASE; FERMENTATION; GENE AMPLIFICATION; GENE DOSAGE; GENE EXPRESSION REGULATION, FUNGAL; METHIONINE; TRANSCRIPTION, GENETIC; TRANSFORMATION, GENETIC;

ACREMONIUM CHRYSOGENUM; CEPHALOSPORIUM;

EID: 0035197231     PISSN: 13675435     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.jim.7000192     Document Type: Article

References (32)

1. Penicillin and cephalosporin biosynthetic genes: Structure, organization, regulation and evolution
2. δ-5-(L-α-Aminoadipyl)-L-cysteinyl-D-valine synthetase, the multi-enzyme integrating the four primary reactions in β-lactam biosynthesis, as a model peptide synthetase
3. Towards a science of metabolic engineering
4. The thioredoxin system of Penicillium chrysogenum and its possible role in penicillin biosynthesis
5. Strain exchange between industry and academy
6. Regulatory properties of O-acetyl-L-serine sulfhydrylase of Cephalosporium acremonium: Evidence of an isoenzyme and its importance in cephalosporin C biosynthesis
7. A spectrophotometric method for the direct determination of cysteine in the presence of other naturally occurring amino acids
8. Expression of the penDE gene of Penicillium chrysogenum encoding isopenicillin N acyltransferase in Cephalosporium acremonium: Production of benzylpenicillin by the transformants
9. Expression of the cefG gene is limiting for cephalosporin biosynthesis in Acremonium chrysogenum
10. δ-(L-α-aminoadipyl)-L-cysteinyl-D-valine synthetase is a rate limiting enzyme for penicillin production in Aspergillus nidulans
11. Targeted inactivation of the mecB gene encoding cystathionine-γ-lyase shows that the transsulfuration pathway is required for high level cephalosporin biosynthesis in Acremonium chrysogenum C10 but not for methionine induction of the cephalosporin genes
12. Characterization of the reverse transsulfuration mecB gene of Acremonium chryogenum encoding a functional, cystathionine-γ-lyase
13. α-Aminoadipyl-cysteinyl-valine synthetases in β-lactam producing organisms. From Abraham's discoveries to novel concepts of non-ribosomal peptide synthesis
14. Molecular control of expression of penicillin biosynthesis genes in fungi: Regulatory proteins interact with a bidirectional promoter region
15. Regulation of biosynthesis of β-lactam antibiotics
16. Organization and expression of genes involved in the biosynthesis of antibiotics and other secondary metabolites
17. Enzymes involved in penicillin, cephalosporin and cephamycin biosynthesis
18. An improved HPLC assay of the substrate and products of the isopenicillin N synthase
19. The biosynthesis of cephalosporin C
20. The biosynthesis of sulfur-containing β-lactam antibiotics
21. Regulation of sulfur amino acid biosynthesis in Aspergillus nidulans: Physiological and genetical aspects
22. Metabolism and functions of glutathionine in microorganisms
23. Coordinate increase of isopenicillin N synthetase, isopenicillin N epimerase and deacetoxycephalosporin C synthetase in a high cephalosporin-producing mutant of A. chrysogenum and simultaneous loss of the three enzymes in a non-producing mutant
24. Cellular thiols and redox-regulated signal transduction
25. Levels of isopenicillin N synthetase and deacetoxycephalosporin C synthetase in Cephalosporium acremonium producing high and low levels of cephalosporin C
26. Dislocation of type I membrane proteins from the ER to the cytosol is sensitive to changes in redox potential
27. Role of sulfur metabolism in cephalosporin C and penicillin biosynthesis
28. Exogenous methionine increase levels of mRNAs transcribed from pcbAB, pcbC, and cefEF genes, encoding enzymes of the cephalosporin biosynthetic pathway, in Acremonium chrysogenum
29. General aspects of secondary metabolism
30. 3′-End processing of pre-mRNA in eukaryotes
31. Carbon catabolite regulation of the conversion of penicillin N into cephalosporin C