Recombinant Thermus aquaticus RNA polymerase, a new tool for structure-based analysis of transcription

Journal of Bacteriology

Volumn 183, Issue 1, 2001, Pages 71-76

  Minakhin, L ^a   Nechaev, S ^a   Campbell, E A ^a   Severinov, K ^a  

^a RUTGERS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RNA POLYMERASE;

ARTICLE; ENZYME ACTIVITY; GENETIC RECOMBINATION; GENETIC TRANSCRIPTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; STRUCTURE ACTIVITY RELATION; THERMUS AQUATICUS;

CLONING, MOLECULAR; DNA-DIRECTED RNA POLYMERASES; ESCHERICHIA COLI; MOLECULAR SEQUENCE DATA; PROMOTER REGIONS (GENETICS); RECOMBINANT PROTEINS; STRUCTURE-ACTIVITY RELATIONSHIP; THERMUS; TRANSCRIPTION, GENETIC;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; THERMUS AQUATICUS;

EID: 0035191283     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.183.1.71-76.2001     Document Type: Article

References (19)

1. Localization of a sigma70 binding site on the N terminus of the Escherichia coli RNA polymerase beta' subunit
2. Recombinant Escherichia coli RNA polymerase: Purification of individually overexpressed subunits and in vitro assembly
3. Studies on the functional topography of Escherichia coli RNA polymerase: Highly selective affinity labeling by analogues of initiating substrates
4. Localisation of the binding site for the initiating substrate on the RNA polymerase from Sulfolobus acidocaldarius
5. Purification and properties of the RNA polymerase of an extremely thermophilic bacterium: Thermus aquaticus T2
6. Bipartite functional map of the E. coli RNA polymerase alpha subunit: Involvement of the C-terminal region in transcription activation by cAMP-CRP
7. Mapping and sequencing of mutations in the Escherichia coli rpoB gene that lead to rifampicin resistance
8. Blocking of the initiation-to-elongation transition by a transdominant RNA polymerase mutation
9. Transcriptional arrest: Escherichia coli RNA polymerase translocates backward, leaving the 3' end of the RNA intact and extruded
10. Determination of intrinsic transcription termination efficiency by RNA polymerase elongation rate
11. Cloning and characterization in Escherichia coli of the gene encoding the principal sigma factor of an extreme thermophile, Thermus thermophilus
12. Active site labeling of the RNA polymerases A, B, and C from yeast
13. Rifampicin region revisited: New rifampicin-resistant and streptolydigin-resistant mutants in the β subunit of Escherichia coli RNA polymerase
14. A mutant RNA polymerase that forms unusual promoter complexes
15. Mutations in and monoclonal antibody binding to evolutionary hypervariable region of E. coli RNA polymerase β' subunit inhibit transcript cleavage and transcript elongation
16. Recombinant RNA polymerase: Inducible overexpression, purification and assembly of Escherichia coli rpo gene products
17. Mapping of catalytic residues in the RNA polymerase active center
18. Crystal structure of Thermus aquaticus core RNA polymerase at 3.3 Å resolution
19. Function and reassembly of subunits of DNA-dependent RNA polymerase