Purification and characterization of novel extracellular endopolygalacturonases from a deep-sea yeast, Cryptococcus sp. N6, isolated from the Japan Trench

Biotechnology Letters

Volumn 23, Issue 21, 2001, Pages 1735-1739

  Miura, Takeshi ^a   Abe, Fumiyoshi ^b   Inoue, Akira ^a,b   Usami, Ron ^a   Horikoshi, Koki ^a,b  

^a TOYO UNIVERSITY   (Japan)

^b JAPAN AGENCY FOR MARINE EARTH SCIENCE AND TECHNOLOGY   (Japan)

Author keywords

Copper tolerant yeast; Cryptococcus; Deep sea yeast; Endopolygalacturonase; Glycoprotein

Indexed keywords

AMINO ACID; ASPARAGINE; GLYCOPEPTIDASE; GLYCOPEPTIDASE F; N ACETYLGLUCOSAMINE; POLYGALACTURONASE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CRYPTOCOCCUS; CULTURE MEDIUM; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME GLYCOSYLATION; ENZYME PURIFICATION; FUSARIUM MONILIFORME; JAPAN; MOLECULAR WEIGHT; NONHUMAN; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; YEAST;

CRYPTOCOCCUS SP.; FUSARIUM; GIBBERELLA FUJIKUROI;

EID: 0035187514     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1012488115482     Document Type: Article

References (12)

1. Endopolygalacturonase production from Kluyveromyces marxianus. I. Resolution, purification and partial characterization of the enzyme
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4. Detection method for polygalacturonase-producing strains of Saccharomyces cerevisiae
5. A rapid and sensitive spectrophotometric method for assaying polygalacturonase using 2-cyanoacetamide
6. Fluorimetric determination of reducing carbohydrates with 2-cyanoacetamide and application to automated analysis of carbohydrates as borate complexes
7. A manual method for the spectrophotometric determination of reducing carbohydrates with 2-cyanoacetamide
8. Co-expression of an Erwinia chrysanthemi pectate lyase-encoding gene (pelE) and an E. carotovora polygalacturonase-encoding gene (phehl) in Saccharomyces cerevisiae
9. Multiple forms of endopolygalacturonase from Saccharomyces fragilius
10. Studies on polygalacturonase of certain yeasts
11. Production of pectic enzymes in yeasts
12. Glycoprotein sttaining following electrophoresis on acrylamide gels