Developmental potential of porcine nuclear transfer embryos derived from transgenic fetal fibroblasts infected with the gene for the green fluorescent protein: Comparison of different fusion/activation conditions

Biology of Reproduction

Volumn 65, Issue 6, 2001, Pages 1681-1685

  Park, K W ^a   Lai, L ^a   Cheong, H T ^a   Im, G S ^a   Sun, Q Y ^a   Wu, G ^a   Day, B N ^a   Prather, R S ^a  

^a UNIVERSITY OF MISSOURI   (United States)

Author keywords

Developmental biology; Early development; Embryo; Gene regulation, ovum

Indexed keywords

CYTOCHALASIN B; GREEN FLUORESCENT PROTEIN;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BLASTOCYST; CELL NUCLEUS TRANSPLANTATION; CONTROLLED STUDY; EMBRYO; EMBRYO DEVELOPMENT; FETUS; FIBROBLAST; IN VITRO STUDY; MICROMANIPULATION; NONHUMAN; OOCYTE CLEAVAGE; PARTHENOGENESIS; PRIORITY JOURNAL; SWINE; TRANSGENIC ANIMAL;

EID: 0035187148     PISSN: 00063363     EISSN: None     Source Type: Journal    
DOI: 10.1095/biolreprod65.6.1681     Document Type: Article

References (16)

1. Development of pig embryos by nuclear transfer of cultured fetal fibroblast cells
2. Development of pig embryos reconstructed by microinjection of cultured fetal fibroblast cells into in vitro matured oocytes
3. Expression of enhanced green fluorescent protein (EGFP) and neomycin resistant (Neo® genes in porcine embryos following nuclear transfer with porcine fetal fibroblasts transfected by retro virus vector
4. In vitro development of reconstructed porcine oocytes after somatic cell nuclear transfer
5. Developmental potential and transgene expression of porcine nuclear transfer embryos using somatic cells
6. Production of transgenic porcine blastocysts by nuclear transfer
7. Cloned pigs produced by nuclear transfer from adult somatic cells
8. Pig cloning by microinjection of fetal fibroblast nuclei
9. Production of cloned pigs from in vitro system
10. Production of cloned swine that express the enhanced green fluorescent protein
11. Culture of pig embryos
12. Transgenic monkeys produced by retroviral gene transfer into mature oocytes
13. Optimization of porcine oocyte activation following nuclear transfer
14. Morphologic evaluation and actin filament distribution in porcine embryos produced in vitro and in vivo
15. Carry-over of mRNA during nuclear transfer in pigs
16. The transition from maternal to zygotic control of development occurs during the 4-cell stage in the domestic pig. Sus scrofa: quantitative and qualitative aspects of protein synthesis