Solution structure of the ras binding domain of the protein kinase byr2 from schizosaccharomyces pombe

Structure

Volumn 9, Issue 11, 2001, Pages 1029-1041

  Gronwald, Wolfram ^a   Huber, Fritz ^a   Grünewald, Petra ^b   Spörner, Michael ^a   Wohlgemuth, Sabine ^b   Herrmann, Christian ^b   Kalbitzer, Hans Robert ^a,b  

^a UNIVERSITY OF REGENSBURG   (Germany)

^b MAX PLANCK INSTITUTE OF MOLECULAR PHYSIOLOGY   (Germany)

Author keywords

Byr2; NMR; Ras binding domain; S. pombe; Signal transduction; Solution structure

Indexed keywords

GUANOSINE TRIPHOSPHATASE; PROTEIN KINASE; PROTEIN KINASE BYR2; RAF PROTEIN; RAS PROTEIN; UBIQUITIN; UNCLASSIFIED DRUG;

ALPHA HELIX; AMINO ACID SEQUENCE; AQUEOUS SOLUTION; ARTICLE; BINDING SITE; COMPLEX FORMATION; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME STRUCTURE; MOLECULAR RECOGNITION; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN FOLDING; PROTEIN LOCALIZATION; PROTEIN MOTIF; PROTEIN PROTEIN INTERACTION; PROTEIN SECONDARY STRUCTURE; SCHIZOSACCHAROMYCES POMBE; SEQUENCE HOMOLOGY; YEAST;

AMINO ACID SEQUENCE; BINDING SITES; ENZYME ACTIVATION; FUNGAL PROTEINS; GUANYLYL IMIDODIPHOSPHATE; MAP KINASE KINASE KINASES; MITOGEN-ACTIVATED PROTEIN KINASES; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN BINDING; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; RAS PROTEINS; SCHIZOSACCHAROMYCES; SCHIZOSACCHAROMYCES POMBE PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

MAMMALIA; SCHIZOSACCHAROMYCES POMBE;

EID: 0035184240     PISSN: 09692126     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0969-2126(01)00671-2     Document Type: Article

References (47)

1. The GTPase superfamily: A conserved switch for diverse cell functions
2. Nuclear magnetic resonance and molecular dynamics studies on the interactions of the Ras-binding domain of Raf-1 with wild-type and mutant Ras proteins
3. Solution structure of the Ras-binding domain of c-Raf-1 and identification of its Ras interaction surface
4. Solution structure of the Ras-binding domain of RGL
5. Structure determination of the Ras-binding domain of the Ral-specific guanine nucleotide exchange factor RLF
6. Structure of the Ras-binding domain of RalGEF and implications for Ras binding and signalling
7. Three-dimensional structure of the Ras-interacting domain of RalGDS
8. Structural and biochemical analysis of Ras-effector signalling via RalGDS
9. Crystal structure and functional analysis of Ras binding to its effector phosphoinositide 3-kinase
10. Protein kinase Byr2 is a target of Ras1 in the fission yeast Schizosaccharomyces pombe
11. Multiple regulatory domains of the Byr2 protein kinase
12. Role of a ras homolog in the life cycle of Schizosaccharomyces pombe
13. Involvement of ras in sexual differentiation but not in growth control in fission yeast
14. Complex formation between RAS and RAF and other protein kinases
15. A gene which encodes a predicted protein kinase can restore some function of the ras gene in fission yeast
16. The ste4+ gene, essential for sexual differentiation of Schizosaccharomyces pombe, encodes a protein with a leucine zipper motif
17. Sam: A novel motif in yeast sterile and Drosophilia polyhomeotic proteins
18. Identification of Ste4 as a potential regulator of Byr2 in the sexual response pathway of Schizosaccharomyces pombe
19. Isolation and characterization of a gene encoding a G-protein alpha subunit from Schizosaccharomyces pombe: Involvement in mating and sporulation pathways
20. Sequential NMR assignment of the Ras-binding domain of Byr2
21. Overcoming the problems associated with poor spectra quality of the protein kinase Byr2 using residual dipole couplings
22. A robust method for determining the magnitude of the fully asymmetric alignment tensor of oriented macromolecules in the absence of structural information
23. Transient kinetic studies on the interaction of Ras and the Ras-binding domain of c-Raf-1 reveal rapid equilibration of the complex
24. Thermodynamic and kinetic characterization of the interaction between the Ras binding domain of AF6 and members of the Ras subfamily
25. Differential interaction of the ras family GTP-binding proteins H-Ras, Rap1A, and R-Ras with the putative effector molecules Raf kinase and Ral-guanine nucleotide exchange factor
26. Conformational transitions in p21ras and its complexes with the effector protein Raf-RBD and the GTPase activating protein GAP
27. Dynamic properties of the Ras switch I region and its importance for binding to effectors
28. Constrained multiple sequence alignment using XALIGN
29. Discrimination of amino acids mediating Ras binding from non interacting residues affecting raf activation by double mutant analysis
30. Quantitative structure activity analysis correlating Ras/Raf interaction in vitro to Raf activation in vivo
31. Analysis of intrinsic and CDC25-stimulated guanine nucleotide exchange of p21ras-nucleotide complexes by fluorescence measurements
32. Direct measurement of distances and angles in biomolecules by NMR in a dilute liquid crystalline medium
33. Improved dilute bicelle solutions for high-resolution NMR of biological macromolecules
34. Characterisation of magnetically oriented phospholipid micelles for measurement of dipolar couplings in macromolecules
35. Attenuated T2 relaxation by mutual cancellation of dipole-dipole coupling and chemical shift anisotropy indicates an avenue to NMR structures of very large biological macromolecules in solution
36. 13C chemical shifts relative to their random coil values
37. AURELIA, a program for computer-aided analysis of multidimensional NMR spectra
38. Relax, a flexible program for the back calculation of NOESY spectra based on complete-relaxation-matrix formalism
39. 15N edited 3D-NOESY-HSQC spectra using back calculated and experimental spectra
40. Crystallography and NMR system: A new software suite for macromolecular structure determination
41. Protein backbone angle restraints from searching a database for chemical shift and sequence homology
42. Molmol: A program for display and analysis of macromolecular structures
43. AQUA and PROCHECK-NMR: Programs for checking the quality of protein structures solved by NMR
44. 15N random coil NMR chemical shifts of the common amino acids. I. Investigations of nearest-neighbor effects
45. Ras/Rap effector specificity determined by charge reversal
46. The Ras-Byr2RBD complex: Structural basis for Ras effector recognition in yeast