Acetylcholinesterase assay for rapid expression screening in liquid and solid media

BioTechniques

Volumn 30, Issue 1, 2001, Pages 81-86

  Villatte, F ^a   Bachman, T T ^a   Hussein, A S ^a   Schmid, R D ^a  

^a UNIVERSITY OF STUTTGART   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ACETYLCHOLINESTERASE; INDOLEACETIC ACID;

ALZHEIMER DISEASE; ANALYTIC METHOD; ARTICLE; BIOSENSOR; ENZYME ASSAY; PICHIA PASTORIS; PROTEIN EXPRESSION; SCREENING;

PICHIA PASTORIS;

EID: 0035176523     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/01301st04     Document Type: Article

References (16)

1. Determination of organophosphorus and carbamate pesticides using a piezoelectric biosensor
2. Colorimetric determination of cholinesterase activity. New methods leading to the formation of soluble and insoluble end-products
3. Acetylcholinesterases of the nematode Steinernema carpocapsae. Characterization of two types of amphiphilic forms differing in their mode of membrane association
4. Improved multianalyte detection of organophosphates and carbamates with disposable multielectrode biosensors using recombinant mutants of Drosophila acetylcholinesterase and artificial neural networks
5. A disposable multielectrode biosensor for rapid simultaneous detection of the insecticides paraoxon and carbofuran at high resolution
6. Biochemical characterization of Drosophila melanogaster acetylcholinesterase expressed by recombinant baculoviruses
7. Production of an immunoenzymatic tracer combining a scFv and the acetylcholinesterase of Bungarus fasciatus by genetic recombination
8. A new and rapid colorimetric determination of acetylcholinesterase activity
9. Resorufin butyrate and indoxyl acetate as fluoregenic substrates for cholinesterase
10. Functional expression of a mammalian acetylcholinesterase in Pichia pastoris: Comparison to acetylcholinesterase, expressed and reconstituted from Escherichia coli
11. Studies in enzyme cytochemistry. An appraisal of indigogenic reactions for esterase localization
12. Cloning, expression and properties of a nonneuronal secreted acetylcholinesterase from the parasitic nematode Nippostrongylus brasiliensis
13. Determinants of substrate specificity of a second non-neuronal secreted acetylcholinesterase from the parasitic nematode Nippostrongylus brasiliensis
14. Expression and processing of vertebrate acetylcholinesterase in the yeast Pichia pastoris
15. A method demonstrating motor endplates for light and electron microscopy
16. Engineering sensitive acetylcholinesterase for detection of organophosphate and carbamate insecticides