Conversion of a glutamate dehydrogenase into methionine/norleucine dehydrogenase by site-directed mutagenesis

European Journal of Biochemistry

Volumn 268, Issue 22, 2001, Pages 5791-5799

  Wang, Xing Guo ^a   Britton, K Linda ^b   Stillman, Timothy J ^b   Rice, David W ^b   Engel, Paul C ^a  

^a UNIVERSITY COLLEGE DUBLIN   (Ireland)

^b UNIVERSITY OF SHEFFIELD   (United Kingdom)

Author keywords

Essential residues; Glutamate dehydrogenase; L methionine dehydrogenase; Site directed mutagenesis; Substrate specificity

Indexed keywords

2 OXOGLUTARIC ACID; ALANINE; AMINO ACID; ASPARTIC ACID; CARBOXYLIC ACID; GLUTAMATE DEHYDROGENASE; GLUTAMIC ACID; LEUCINE; LEUCINE DEHYDROGENASE; METHIONINE DEHYDROGENASE; NORLEUCINE DEHYDROGENASE; NORVALINE; OXIDOREDUCTASE; UNCLASSIFIED DRUG; VALINE;

ARTICLE; CATALYSIS; CHROMATOGRAPHY; CONTROLLED STUDY; DEAMINATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME BINDING; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE COMPLEX; MUTATION; NONHUMAN; PH; PRIORITY JOURNAL; REACTION ANALYSIS; SITE DIRECTED MUTAGENESIS;

AMINO ACID OXIDOREDUCTASES; BASE SEQUENCE; COENZYMES; DNA PRIMERS; FORMAZANS; GLUTAMATE DEHYDROGENASE; INDICATORS AND REAGENTS; KINETICS; MUTAGENESIS, SITE-DIRECTED; PROTEIN BINDING; SUBSTRATE SPECIFICITY;

EID: 0035174871     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.0014-2956.2001.02523.x     Document Type: Article

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