A new expression cloning strategy for isolation of substrate-specific kinases by using phosphorylation site-specific antibody

Journal of Immunological Methods

Volumn 247, Issue 1-2, 2001, Pages 141-151

  Matsuo, Ritsuko ^a   Ochiai, Wataru ^a,b   Nakashima, Kinichi ^a,b   Taga, Tetsuya ^a,b  

^a TOKYO MEDICAL AND DENTAL UNIVERSITY   (Japan)

^b KUMAMOTO UNIVERSITY   (Japan)

Author keywords

DYRK; HIPK2; Immunoscreening; Serine threonine kinase; STAT3

Indexed keywords

GLUTATHIONE TRANSFERASE; MITOGEN ACTIVATED PROTEIN KINASE; PHOSPHOTRANSFERASE; PROTEIN SERINE THREONINE KINASE; SERINE; STAT3 PROTEIN;

ANIMAL CELL; ANTIBODY SPECIFICITY; ARTICLE; DNA LIBRARY; ENZYME ISOLATION; ENZYME PHOSPHORYLATION; FEMALE; MAMMAL CELL; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SCREENING; SIGNAL TRANSDUCTION;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES; ANTIBODY SPECIFICITY; BINDING SITES; CARRIER PROTEINS; CERCOPITHECUS AETHIOPS; CLONING, MOLECULAR; COS CELLS; DNA-BINDING PROTEINS; FEMALE; GENE EXPRESSION; INTERLEUKIN-6; MITOGEN-ACTIVATED PROTEIN KINASE 1; MITOGEN-ACTIVATED PROTEIN KINASE 3; MITOGEN-ACTIVATED PROTEIN KINASES; MOLECULAR SEQUENCE DATA; PHOSPHORYLATION; PHOSPHOSERINE; PROTEIN KINASES; PROTEIN-SERINE-THREONINE KINASES; PROTEIN-TYROSINE KINASES; RABBITS; STAT3 TRANSCRIPTION FACTOR; SUBSTRATE SPECIFICITY; TRANS-ACTIVATORS;

EID: 0035174668     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(00)00313-6     Document Type: Article

References (30)

1. Molecular cloning of APRF, a novel IFN-stimulated gene factor involved in the gp130-mediated signaling pathway
2. Sequence characteristics, subcellular localization, and substrate specificity of DYRK-related kinases, a novel family of dual specificity protein kinases
3. STAT3 activation by cytokines utilizing gp130 and related transducers involves a secondary modification requiring an H7-sensitive kinase
4. The homeodomain protein NK-3 recruits Groucho and a histone deacetylase complex to repress transcription
5. STAT3 serine phosphorylation by ERK-dependent and -independent pathways negatively modulates its tyrosine phosphorylation
6. Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins
7. STATs and gene regulation
8. Two signals are necessary for cell proliferation induced by a cytokine receptor gp130: Involvement of STAT3 in anti-apoptosis
9. Organization and regulation of mitogen-activated protein kinase signaling pathways
10. Identification of substrate recognition determinants for human ERK1 and ERK2 protein kinases
11. The protein kinase family: Conserved features and deduced phylogeny of the catalytic domains
12. Protein kinase catalytic domain sequence database: Identification of conserved features of primary structure and classification of family members
13. TGF-β signalling from cell membrane to nucleus through SMAD proteins
14. Cytokine receptor signalling
15. Choline/ethanolamine kinase from mammalian tissues
16. Protein kinase Cδ associates with and phosphorylates Stat3 in an Interleukin-6-dependent manner
17. Dyrk, a dual specificity protein kinase with unique structural features whose activity is dependent on tyrosine residues between subdomains VII and VIII
18. Homeodomain-interacting protein kinases, a novel family of co-repressors for homeodomain transcription factors
19. IL-6-inducible complexes on an IL-6 response element of the junB promoter contain Stat3 and 36 kDa CRE-like site binding protein(s)
20. Extracellular signal-regulated protein kinase (ERK)-dependent and ERK-independent pathways target STAT3 on serine-727 in human neutrophils stimulated by chemotactic factors and cytokines
21. Interleukin-induced serine phosphorylation of transcription factor APRF: Evidence for a role in interleukin-6 target gene induction
22. Controlling TGF-β signaling
23. Specificity of receptor tyrosine kinase signaling: Transient versus sustained extracellular signal-regulated kinase activation
24. STAT3 activation is a critical step in gp130-mediated terminal differentiation and growth arrest of a myeloid cell line
25. Signal perception and transduction: The role of protein kinase
26. Growth factor signaling by receptor tyrosine kinases
27. Choice of STATs and other substrates specified by modular tyrosine-based motifs in cytokine receptors
28. GP130 and the interleukin-6 family of cytokines
29. Maximal activation of transcription by Stat1 and Stat3 requires both tyrosine and serine phosphorylation
30. Mapping of Stat3 serine phosphorylation to a single residue(727) and evidence that serine phosphorylation has no influence on DNA binding of Stat1 and Stat3