Characterization of two DNA polymerases from the hyperthermophilic euryarchaeon Pyrococcus abyssi

European Journal of Biochemistry

Volumn 268, Issue 22, 2001, Pages 5961-5969

  Gueguen, Yannick ^a,c   Rolland, Jean Luc ^a   Lecompte, Odile ^b   Azam, Philippe ^a   Le Romancer, Gisèle ^a   Flament, Didier ^a   Raffin, Jean Paul ^a   Dietrich, Jacques ^a  

^a IFREMER   (France)

^b INSTITUT DE GÉNÉTIQUE ET DE BIOLOGIE MOLÉCULAIRE ET CELLULAIRE   (France)

^c Defense et Resistanc'e chez les Invertebres Marins (DRIM)   (France)

Author keywords

Archaea; DNA polymerase; DNA replication; Exonuclease activity; Pyrococcus abyssi

Indexed keywords

DNA DIRECTED DNA POLYMERASE ALPHA; DNA DIRECTED DNA POLYMERASE BETA; EXONUCLEASE;

AMINO ACID SEQUENCE; ARTICLE; CATALYSIS; CONTROLLED STUDY; DNA REPLICATION; ENZYME ACTIVITY; ENZYME STRUCTURE; ENZYME SUBUNIT; ESCHERICHIA COLI; GENE EXPRESSION; GENE LOCATION; GENE SEQUENCE; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; PH; PRIORITY JOURNAL; PYROCOCCUS ABYSSI; SEQUENCE ANALYSIS;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; DNA-DIRECTED DNA POLYMERASE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME STABILITY; HYDROGEN-ION CONCENTRATION; MOLECULAR SEQUENCE DATA; PYROCOCCUS; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY;

EID: 0035171927     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.0014-2956.2001.02550.x     Document Type: Article

References (35)

1. Proliferating cell nuclear antigen: More than a clamp for DNA polymerases
2. Towards a natural system of organisms: Proposal for the domains archaea, bacteria, and eucarya
3. Cloning and characterization of a family B DNA polymerase from the hyperthermophilic crenarchaeon Pyrobaculum islandicum
4. Two DNA polymerase sliding clamps from the thermophilic archaeon Sulfolobus solfataricus
5. Complete genome sequence of the methanogenic Archaeon, Methanococcus jannaschii
6. The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus
7. Complete sequence of the gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3
8. Archaeal DNA replication: Identifying the pieces to solve a puzzle
9. Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1
10. Mapping of a chromosome replication origin in an archaeon: Response
11. Two family B DNA polymerases from Aeropyrum pernix, an aerobic hyperthermophilic crenarchaeote
12. The hyperthermophilic archaeon Pyrodictium occultum has two alpha-like DNA polymerases
13. Gene duplications in evolution of archaeal family B DNA polymerases
14. Pyrococcus abyssi sp. nov., a new hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent
15. DNA and protein engineering using the polymerase chain reaction: Splicing by overlap extension
16. Stabilization of T7-promoter-based pARHS expression vectors using the parB locus
17. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
18. A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of protein-dye binding
19. The PCNA from Thermococcus fumicolans functionally interacts with DNA polymerase delta
20. Characterization of a DNA polymerase from the hyperthermophile Archae Thermococcus litoralis. Vent DNA polymerase, steady state kinetics, thermal stability, processivity, strand displacement, and exo-nuclease activities
21. Protein splicing: Inteins and exteins - A definition of terms and recommended nomenclature
22. Bacterial mode of replication with eukaryotic-like machinery in a hyperthermophilic archaeon
23. The replication origin of archaea is finally revealed
24. A novel family of DNA-polymerase-associated B subunits
25. A heterodimeric DNA polymerase: Evidence that members of Euryarchaeota possess a distinct DNA polymerase
26. Invariant Asp1122 and Asp1124 are essential residues for polymerization catalysis of family D DNA polymerase from Pyrococcus horikoshii
27. A novel DNA polymerase in the hyperthermophilic archaeon, Pyrococcus furiosus: Gene cloning, expression and characterization
28. A novel DNA polymerase family found in Archaea
29. A general structure for DNA-dependent DNA polymerases
30. Phosphodiestarase domains associated with DNA polymerases of diverse origins
31. Protein serine/threonine phosphatases
32. Structural biochemistry an interaction architecture of the DNA double-stand break repair Mre11 nuclease and Rad50-ATPase
33. Functional interactions of a homolog of proliferating cell nuclear antigen with DNA polymerases in Archaea
34. DbClustal: Rapid and reliable global multiple alignments of protein sequences detected by database searches