Lysis of whole blood in vitro causes DNA strand breaks in human lymphocytes

Mutagenesis

Volumn 16, Issue 6, 2001, Pages 455-459

  Narayanan, S ^a   O'Donovan, M R ^b   Duthie, S J ^a  

^a ROWETT RESEARCH INSTITUTE   (United Kingdom)

^b ASTRAZENECA   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ANALYSIS; ARTICLE; BIOLOGICAL MONITORING; BLOOD CULTURE; COMET ASSAY; CONTROLLED STUDY; CYTOLYSIS; DNA DAMAGE; DNA STRAND BREAKAGE; GEL ELECTROPHORESIS; GENOTOXICITY; GRANULOCYTE; HUMAN; HUMAN CELL; IN VITRO STUDY; LYMPHOCYTE; LYMPHOCYTE ACTIVATION; LYMPHOCYTE CULTURE; METHODOLOGY; PERIPHERAL LYMPHOCYTE; PH; POPULATION; PRIORITY JOURNAL; SAMPLING; TIME;

EID: 0035170812     PISSN: 02678357     EISSN: None     Source Type: Journal    
DOI: 10.1093/mutage/16.6.455     Document Type: Article

References (21)

1. Effects in the comet assay of storage conditions on human blood
2. 8-Nitro-2′-deoxyguanosine, a specific marker of oxidation by reactive nitrogen species, is generated by the myeloperoxidase-hydrogen peroxide-nitrite system of activated human phagocytes
3. Somatic mutant frequency, mutation rates and mutational spectra in the human population in vivo
4. Direct enzymic detection of endogenous oxidative base damage in human lymphocyte DNA
5. Platelets as scavengers of neutrophil-derived oxidants: A possible defence mechanism at sites of vascular injury
6. Uracil misincorporation in human DNA detected using single cell gel electrophoresis
7. UV-C sensitivity of unstimulated and stimulated human lymphocytes from normal and xeroderma pigmentosum donors in the comet assay: A potential diagnostic technique
8. Effect of deoxyribonucleosides on the hypersensitivity of human peripheral blood lymphocytes to UV-B and UV-C irradiation
9. Properties, inducibility and an improved method of analysis of aryl hydrocarbon hydroxylase in cultured human lymphocytes
10. Levels of peripheral blood cell DNA damage in insulin dependent diabetes mellitus human subjects
11. Blood storage and the rate of chromosome aberrations after in vitro exposure to ionising radiations
12. Activated murine neutrophils induce unscheduled DNA synthesis in B lymphocytes
13. Effect of blood storage on radiation-induced micronuclei in human lymphocytes
14. The single cell gel electrophoresis assay (comet assay): A European review
15. Extended-term cultures of human T-lymphocytes: A practical alternative to primary human lymphocytes for use in genotoxicity testing
16. The effect of PHA stimulation on lymphocyte sub-populations in whole blood cultures
17. Heterogeneity in radiation-induced DNA damage and repair in tumor and normal cells measured using the 'comet' assay
18. Biomarkers and molecular epidemiology in mutation/cancer research
19. Activated neutrophils induce prolonged DNA damage in neighbouring cells
20. An analysis of X-ray-induced DNA damage in human blood leukocytes, lymphocytes and granulocytes