Successful PCR amplification of genomic DNA from Cryptosporidium parvum oocysts extracted from a human faecal sample: A rapid and simple method suited for outbreak analysis

International Journal of Hygiene and Environmental Health

Volumn 204, Issue 2-3, 2001, Pages 191-194

  Millar, Cherie ^a   Moore, John ^a   Lowery, Colm ^a,b   McCorry, Kieran ^a   Dooley, James ^b  

^a BELFAST CITY HOSPITAL   (United Kingdom)

^b UNIVERSITY OF ULSTER   (United Kingdom)

Author keywords

Cryptosporidium parvum; DNA extraction; Faeces; PCR

Indexed keywords

PROTOZOAL DNA;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; CRYPTOSPORIDIUM PARVUM; EPIDEMIC; EXTRACTION; FECES ANALYSIS; GENE AMPLIFICATION; GENE ISOLATION; GENE LOCUS; NONHUMAN; OOCYST; POLYMERASE CHAIN REACTION; TECHNIQUE;

ANIMALIA; CRYPTOSPORIDIUM PARVUM; PROTOZOA;

EID: 0035169714     PISSN: 14384639     EISSN: None     Source Type: Journal    
DOI: 10.1078/1438-4639-00090     Document Type: Article

References (10)

1. A microsatellite marker reveals population heterogeneity within human and animal genotypes of Cryptosporidium parvum
2. Laboratory diagnosis of cryptosporidiosis
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5. Simplified method for recovery and PCR detection of Cryptosporidium DNA from bovine feces
6. Detection and speciation of Cryptosporidium spp. In environmental water samples by immunomagnetic separation, PCR and endonuclease restriction
7. A simple and sensitive method to extract bacterial, yeast and fungal DNA from blood culture material
8. Differentiating human from animal isolates of Cryptosporidium parvum
9. Detection of genes in feces by booster polymerase chain reaction
10. Threshold of detection of Cryptosporidium oocysts in human stool specimens: Evidence for low sensitivity of current diagnostic methods