Glutamine replenishment and ammonia removal in hybridoma cell cultures via immobilized glutamine synthetase

Biochemical Engineering Journal

Volumn 9, Issue 2, 2001, Pages 125-133

  Okeson, Carl D ^a   Riley, Mark R ^a  

^a University of Arizona   (United States)

Author keywords

Ammonia removal; Bioreactors; Enzyme activity; Glutamine synthetase; Hybridoma cultures; Immobilized enzymes

Indexed keywords

AMMONIA; GLUTAMATE AMMONIA LIGASE; GLUTAMIC ACID; GLUTAMINE; MAGNESIUM;

ANIMAL CELL; ARTICLE; CELL CULTURE; CONTROLLED STUDY; CULTURE MEDIUM; DILUTION; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; HYBRIDOMA; NONHUMAN; PRIORITY JOURNAL; THERMOSTABILITY;

EID: 0035167772     PISSN: 1369703X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1369-703X(01)00134-6     Document Type: Article

References (20)

1. A kinetic analysis of hybridoma growth and metabolism in batch and continuous suspension culture: Effect of nutrient concentration, dilution rate, and pH
2. Growth, metabolic, and antibody production kinetics of hybridoma cell culture. 1. Analysis of data from controlled batch reactors
3. Effects of ammonia and lactate on hybridoma growth, metabolism, and antibody production
4. Factors affecting monoclonal antibody production in culture
5. Kinetic studies and unstructured models of lymphocyte metabolism in fed-batch culture
6. Methods for reducing the ammonia in hybridoma cell cultures
7. HBs-mAb production in perfusion culture with selective ammonia removal system
8. Regulation of glutamine synthetase. 1. Purification and properties of glutamine synthetase from Escherichia coli
9. Modification of glucose and glutamine metabolism in hybridoma cells through metabolic engineering
10. High-level expression of a recombinant antibody from myeloma cells using a glutamine synthetase gene as an amplifiable selectable marker
11. Variation of the conformational states of Escherichia coli glutamine synthetase by interaction with different divalent cations
12. Effect of metal ions and adenylylation state on the internal thermodynamics of phosphoryl transfer in the Escherichia coli glutamine synthetase reaction
13. Usefulness of immobilized glutamine synthetase for the removal of ammonia and its application to the determination of creatinine in urine
14. Estimation of ammonia removal activity of glutamine synthetase immobilized gel with the aim of selective detoxification of blood ammonia
15. Production of monoclonal antibodies by agarose-entrapped hybridoma cells