Biosynthesis of the cancer-related sialyl-α2,6-lactosaminyl epitope in colon cancer cell lines expressing β-galactoside α2,6-sialyltransferase under a constitutive promoter

European Journal of Biochemistry

Volumn 268, Issue 22, 2001, Pages 5876-5884

  Dall'Olio, Fabio ^a,c   Chiricolo, Mariella ^a   Mariani, Erminia ^a,b   Facchini, Andrea ^a,b  

^a UNIVERSITY OF BOLOGNA   (Italy)

^b RIZZOLI ORTHOPAEDIC INSTITUTE   (Italy)

^c Dipartimento di Patologia Sperimentale   (Italy)

Author keywords

2,6 sialyltransferase; CDw75; Colon cancer; Glycosylation; Glycosyltransferases

Indexed keywords

BETA GALACTOSIDE ALPHA 2,6 SIALYLTRANSFERASE; COMPLEMENTARY DNA; EPITOPE; IMMUNOGLOBULIN ANTIBODY; LECTIN; MESSENGER RNA; SIALYLTRANSFERASE; UNCLASSIFIED DRUG;

ARTICLE; BIOSYNTHESIS; CANCER CELL CULTURE; CELL SURFACE; CELL TYPE; COLON CARCINOMA; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME RELEASE; ERYTHRINA; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; MEASUREMENT; MOLECULE; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN PROCESSING; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SIALYLATION; TIME;

BASE SEQUENCE; BLOTTING, WESTERN; CELL SEPARATION; COLONIC NEOPLASMS; DNA PRIMERS; EPITOPES; FLOW CYTOMETRY; HUMANS; PROMOTER REGIONS (GENETICS); RNA, MESSENGER; SIALYLTRANSFERASES; TUMOR CELLS, CULTURED;

EID: 0035167142     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.0014-2956.2001.02536.x     Document Type: Article

References (38)

1. Up-regulation of a set of glycosyltransferase genes in human colorectal cancer
2. Alterations of O-glycan biosynthesis in human colon cancer tissues
3. Sialylation of glycoprotein oligosaccharides N-linked to asparagine. Enzymatic characterization of a Galβ1,3(4)GlcNAc α2,3-sialyltransferase and a Galβ1,4GlcNAc α2,6-sialyltransferase from rat liver
4. The sialyl-α2,6-lactosaminyl-structure: Biosynthesis and functional role
5. Increased CMP-NeuAc: Galβ1,4GlcNAc-R α2,6-sialyltransferase activity in human colorectal cancer tissues
6. Enhanced activity of CMP-neuAc: Galβ1-4GlcNAc: A 2,6-sialyltransferase in metastasizing human colorectal tumor tissue and serum of tumor patients
7. Clinical correlations of α2,6-sialyltransferase expression in colorectal cancer patients
8. Expression of α2,6-sialylated sugar chains in normal and neoplastic colon tissues. Detection by digoxigenin-conjugated Sambucus nigra agglutinin
9. Expression of α 2,6-linked sialic acid residues in neoplastic but not in normal human colonic mucosa. A lectin-gold cytochemical study with Sambucus nigra and Maackia amurensis lectins
10. Expression of Sia α2→6Galβ1→4GlcNAc residues on sugar chains of glycoproteins including carcinoembryonic antigens in human colon adenocarcinoma: Applications of Trichosanthes japonica agglutinin I for early diagnosis
11. Quantitative lectin-histochemical and immunohistochemical studies on the occurrence of α(2,3)- and α(2,6)-linked sialic acid residues in colorectal carcinomas. Relation to clinicopathologic features
12. Differential expression of the hepatic transcript of β-galactoside α2,6-sialyltransferase in human colon cancer cell lines
13. β-Galactoside α2,6-sialyltransferase in human colon cancer: Contribution of multiple transcripts to regulation of enzyme activity and reactivity with Sambucus nigra agglutinin
14. Altered mRNA expression of glycosyltransferases in human colorectal carcinomas and liver metastases
15. Classification of human colorectal adenocarcinoma cell lines
16. Enhanced CMP-NeuAc: Galβ1,4GlcNAc-R α2,6-sialyltransferase activity of human colon cancer xenografts in athymic nude mice and of xenograft-derived cell lines
17. Human colon cancer cell lines permanently expressing α2,6- sialylated sugar chains by transfection with rat β-galactoside α2,6-sialyltransferase cDNA
18. Differentiation -dependent expression of human β-galactoside α2,6-sialyltransferase mRNA in colon carcinoma CaCo-2 cells
19. Differential expression of β1,3galactosyltransferases in human colon cells derived from adenocarcinomas or normal mucosa
20. The elderberry (Sambucus nigra L.) bark lectin recognizes the Neu5Ac (α2-6)Gal/GalNAc sequence
21. Purification and properties of a D-galactose/N-acetyl-D-galactosamine- specific lectin from Erythrina cristagalli
22. Branch specificity of bovine colostrum CMP-sialic acid: Galβ1,4GlcNAc-R α2,6-sialyltransferase. Sialylation of bi-, tri-, and tetraantennary oligosaccharides and glycopeptides of the N-acetyllactosamine type
23. Mitogenic leukoagglutinin from Phaseolus vulgaris binds to a pentasaccharide unit in N-acetyllactosamine-type glycoprotein glycans
24. The HB-6, CDw75, and CD76 differentiation antigens are unique cell- surface carbohydrate determinants generated by the β-galactoside α2,6-sialyltransferase
25. Branch specificity of bovine colostrum CMP-sialic acid: N-acetyllactosaminide α2,6-sialyltransferase. Interaction with biantennary oligosaccharides and glycopeptides of N-glycosylproteins
26. CDw75 antigen expression in human gastric carcinoma and adjacent mucosa
27. Differential expression of α2-6 sialylated polylactosamine structures by human B and T cells
28. Minimal structural and glycosylation requirements for ST6Gal I activity and trafficking
29. The role of the carbohydrate chains of Galβ-1,4-GlcNAc α2,6-sialyltransferase for enzyme activity
30. A disulfide-bonded dimer of the Golgi β-galactoside α2,6-sialyltransferase is catalytically inactive yet still retains the ability to bind galactose
31. Two naturally occurring α2,6-sialyltransferase forms with a single amino acid change in the catalytic domain differ in their catalytic activity and proteolytic processing
32. Sialyltransferase isoforms are phosphorylated in the cis-medial Golgi on serine and threonine residues in their luminal sequences
33. Biosynthesis and intracellular transport of α-2,6-sialyltransferase in rat hepatoma cells
34. Studies on the effect of inflammation on rat liver and serum sialyltransferase. Evidence that inflammation causes release of Galβ1,4GlcNAc α2,6-sialyltransferase from liver
35. The expression of soluble and cell-bound α2,6-sialyltransferase in human colonic carcinoma CaCo-2 cells correlates with the degree of enterocytic differentiation
36. 2-terminal signal anchor with a signal peptide
37. 2-terminal signal anchor
38. Two soluble glycosyltransferases glycosylate less efficiently in vivo than their membrane bound counterparts