The application of the restriction site mutation assay to compare 1-ethyl-1-nitrosourea-induced mutations between the endogenous p53 gene and the transgenic LacZ gene in MutaMouse testes

Mutagenesis

Volumn 16, Issue 1, 2001, Pages 59-64

  Song, Hong Lin ^a   Jenkins, Gareth J S ^b   Ashby, John ^c   Tinwell, Helen ^c   Parry, James M ^b  

^a ABERYSTWYTH UNIVERSITY   (United Kingdom)

^b SWANSEA UNIVERSITY   (United Kingdom)

^c ASTRAZENECA   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BETA GALACTOSIDASE; DNA; ETHYLNITROSOUREA; NUCLEIC ACID; PROTEIN P53;

ACCURACY; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; ASSAY; CHEMICAL MUTAGENESIS; COMPARATIVE STUDY; CONTROLLED STUDY; EXON; GENE LOCUS; GENE MUTATION; GENE TARGETING; GENETIC ANALYSIS; GENETIC SELECTION; INTRON; MOUSE; MUTATION RATE; NONHUMAN; PRIORITY JOURNAL; RESTRICTION SITE; SPONTANEOUS MUTATION; TESTIS; TRANSGENIC MOUSE;

ANIMALIA; MUS MUSCULUS; RODENTIA;

EID: 0035163880     PISSN: 02678357     EISSN: None     Source Type: Journal    
DOI: 10.1093/mutage/16.1.59     Document Type: Article

References (31)

1. Mutation assays in male germ cells from transgenic mice: Overview of study and conclusions
2. Quantitative determination of mRNA phenotypes by the polymerase chain reaction
3. TMMouse)
4. Heterogeneous DNA damage and repair in the mammalian genome
5. A comparison of effects of diverse mutagens at lacZ transgene and Dlb-1 locus in vivo
6. Temporal and molecular characteristics of mutations induced by ethylnitrosourea in germ cells isolated from seminiferous tubules and in spermatozoa of LacZ transgenic mice
7. Toward an understanding of the use of transgenic mice for the detection of gene mutations in germ cells
8. Transgenic mice as model systems for studying gene mutations in vivo
9. Efficient rescue of integrated shuttle vectors from transgenic mice: A model for studying mutations in vivo
10. Somatic point mutations in the p53 gene of human tumours and cell lines: Update compilation
11. TMMouse: A transgenic mouse model
12. Mutation analysis using the restriction site mutation (RSM) assay
13. Enhanced restriction site mutation (RSM) analysis of 1,2-dimethylhydrazine induced mutations, using endogenous p53 intron sequences
14. The restriction site mutation assay: A review of the methodology development and the current status of the technique
15. Studies on mutations in male germ cells of transgenic mice following exposure to isopropyl methanesulfonate, ethylnitrosourea or X-ray
16. Development of a short-term, in vivo mutagenesis assay: The effects of methylation on the recovery of lambda phage shuttle vector from transgenic mice
17. Cancer: p53, guardian of the genome
18. Comparison of the in vivo mutagenesis in the endogenous Hprt gene and the LacI transgene of BigBlue rats treated with 7,12-dimthylbenz[a]anthracene
19. TMMice
20. A simple salting out procedure for extracting DNA from human nucleated cells
21. TMMouse: A transgenic mouse model for detecting mutations in vivo
22. An alternative quantitative polymerase chain reaction method
23. TMMouse germ cells (seminiferous tubles cells and epididymis spermatozoa)
24. Enhanced somatic mutation rates induced in stem cells by low chronic exposures to ethylnitrosourea
25. TM B6C3F1 mice
26. Development of new molecular procedures for the detection of genetic alteration in man
27. Comparison of somatic mutation in a transgenic versus host locus
28. TMmouse positive selection transgenic mutation
29. Murine tumour suppressor models
30. Transgenic assays for mutations and cancer: Current status and future perspectives
31. Wild type p53 induced apoptosis of myeloid leukaemic cells that is inhibited by interleukin 6