Assessment of the proportion of transgene-bearing sperm by fluorescence in situ hybridization: A novel approach for the detection of germline mosaicism in transgenic male founders

Molecular Reproduction and Development

Volumn 58, Issue 2, 2001, Pages 166-172

  Ibáñez, Elena ^a   Molist, Josep ^a   Vidal, Francesca ^a   Egozcue, Josep ^a   Santal, Josep ^a  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

Author keywords

FISH; Livestock; Spermatozoa; Transgenic mice

Indexed keywords

ACCURACY; ANALYTIC METHOD; ANIMAL CELL; ARTICLE; BREEDING; FLUORESCENCE IN SITU HYBRIDIZATION; GENETIC SCREENING; GERM LINE; MALE; MOSAICISM; MOUSE; NONHUMAN; PRIORITY JOURNAL; RELIABILITY; SPERMATOZOON; TRANSGENE; TRANSGENIC MOUSE;

ANIMALS; FOUNDER EFFECT; IN SITU HYBRIDIZATION, FLUORESCENCE; LACTOGLOBULINS; MALE; MICE; MICE, INBRED C57BL; MICE, TRANSGENIC; MOSAICISM; SENSITIVITY AND SPECIFICITY; SPERMATOZOA; TRANSGENES;

EID: 0035163211     PISSN: 1040452X     EISSN: None     Source Type: Journal    
DOI: 10.1002/1098-2795(200102)58:2<166::AID-MRD5>3.0.CO;2-Y     Document Type: Article

References (23)

1. Transgenic cattle resulting from biopsied embryos: Expression of c-ski in a transgenic calf
2. Fate of microinjected genes in preimplantation mouse embryos
3. Transgenic livestock as bioreactors: Stable expression of human alpha-1-antitrypsin by a flock of sheep
4. Timing of DNA integration, transgenic mosaicism, and pronuclear microinjection
5. Fluorescence in situ hybridization of interphase nuclei isolated from whole blood of transgenic mice
6. Production and breeding of transgenic cattle using in vitro embryo production technology
7. Oligonucleotide-primed in situ DNA synthesis (PRINS): A method for chromosome mapping, banding, and investigation of sequence organization
8. Simultaneous detection of X- and Y-bearing bull spermatozoa by double colour Fluorescence in situ hybridization
9. Generation of transgenic female offspring for a highly mosaic lactoferrin founder bull using a MOET-biopsy-PCR approach
10. Manipulating the mouse embryo. A laboratory manual, 2nd edition
11. Generation of transgenic dairy cattle from transgene-analyzed and sexed embryos produced in vitro
12. Expression of caprine β-lactoglobulin in the milk of transgenic mice
13. Selection of transgenic preimplantation mouse embryos using fluorescence in situ hybridization
14. Birth of successfully identified transgenic goats using preimplantation stage embryos biopsied for FISH
15. Preservation of hamster oocytes to assay the fertilizing capacity of human spermatozoa
16. Resource requirements for transgenic livestock production
17. The LacZ transgene in MutaTM Mouse maps to chromosome 3
18. High-level expression of a heterologous protein in the milk of transgenic swine using the cDNA encoding human protein C
19. Transgenic livestock: Progress and prospects for the future
20. High-level synthesis of a heterologous milk protein in the mammary glands of transgenic swine
21. Chromosomal mapping of human angiotensinogen gene and human renin gene by Fluorescence in situ hybridization (FISH) in transgenic mice
22. 0 transgenic mice are derived from mosaic embryos
23. Germline and somatic mosaicism in transgenic mice