Reducing the native tropism of adenovirus vectors requires removal of both CAR and integrin interactions

Journal of Virology

Volumn 75, Issue 23, 2001, Pages 11284-11291

  Einfeld, D A ^a   Schroeder, R ^a   Roelvink, P W ^a   Lizonova, A ^a   King, C R ^a   Kovesdi, I ^a   Wlckham, T J ^a  

^a GENVEC INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COAT PROTEIN; DNA; INTEGRIN; VIRUS PROTEIN; VIRUS VECTOR;

ADENOVIRUS; ANIMAL CELL; ARTICLE; GENE THERAPY; GENE TRANSFER; GENETIC TRANSDUCTION; HUMAN; HUMAN CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN PROTEIN INTERACTION; VIRUS GENOME;

ADENOVIRIDAE; ANIMALS; BASE SEQUENCE; CELL ADHESION MOLECULES; CELL LINE; DNA PRIMERS; GENETIC VECTORS; HUMANS; INTEGRINS; POLYMERASE CHAIN REACTION; TRANSFECTION; TROPISM;

EID: 0035158631     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.75.23.11284-11291.2001     Document Type: Article

References (33)

1. Blood clearance rates of adenovirus type 5 in mice
2. Cancer treatment by targeted drug delivery to tumor vasculature in a mouse model
3. Mutations that alter an Arg-Gly-Asp (RGD) sequence in the adenovirus type 2 penton base protein abolish its cell-rounding activity and delay virus reproduction in flat cells
4. The murine CAR homolog is a receptor for coxsackie B viruses and adenoviruses
5. Modulation of Starling forces and muscle fiber maturity permits adenovirus-mediated gene transfer to adult dystrophic (mdx) mice by the intravascular route
6. Construction of a pseudoreceptor that mediates transduction by adenoviruses expressing a ligand in fiber or penton base
7. Expression of coxsackie adenovirus receptor and alphav-integrin does not correlate with adenovector targeting in vivo indicating anatomical vector barriers
8. Gradient of RGD-dependent entry of adenoviral vector in nasal and intrapulmonary epithelia: Implications for gene therapy of cystic fibrosis
9. Fibroblast growth factor 2 retargeted adenovirus has redirected cellular tropism: Evidence for reduced toxicity and enhanced antitumor activity in mice
10. The presence of human coxsackievirus and adenovirus receptor is associated with efficient adenovirus-mediated transgene expression in human melanoma cell cultures
11. Adenoviral-mediated gene transfer in lymphocytes
12. The role of Kupffer cell activation and viral gene expression in early liver toxicity after infusion of recombinant adenovirus vectors
13. Highly efficient adenovirus-mediated gene transfer to cardiac myocytes after single-pass coronary delivery Hum
14. Role of alpha(v) integrins in adenovirus cell entry and gene delivery
15. Vascular targeting with phage peptide libraries
16. Limited entry of adenovirus vectors into well-differentiated airway epithelium is responsible for inefficient gene transfer
17. A targetable, injectable adenoviral vector for selective gene delivery to pulmonary endothelium in vivo
18. Comparative analysis of adenovirus fiber-cell interaction: Adenovirus type 2 (Ad2) and Ad9 utilize the same cellular fiber receptor but use different binding strategies for attachment
19. The coxsackievirus-adenovirus receptor protein can function as a cellular attachment protein for adenovirus serotypes from subgroups A, C, D, E, and F. J.
20. Identification of a conserved receptor-binding site on the fiber proteins of CAR-recognizing adenoviridae
21. Sequestration of adenoviral vector by kupffer cells leads to a nonlinear dose response of transduction in liver
22. HCAR and MCAR: The human and mouse cellular receptors for subgroup C adenoviruses and group B coxsackieviruses
23. Molecular adaptors for vascular-targeted adenoviral gene delivery
24. Basolateral localization of fiber receptors limits adenovirus infection from the apical surface of airway epithelia
25. Increasing epithelial junction permeability enhances gene transfer to airway epithelia in vivo
26. Targeting of adenovirus penton base to new receptors through replacement of its RGD motif with other receptor-specific peptide motifs
27. Integrins alpha v beta 3 and alpha v beta 5 promote adenovirus internalization but not virus attachment
28. Increased in vitro and in vivo gene transfer by adenovirus vectors containing chimeric fiber proteins
29. Enhancement of in vivo adenovirus-mediated gene transfer and expression by prior depletion of tissue macrophages in the target organ
30. Innate immune mechanisms dominate elimination of adenoviral vectors following in vivo administration
31. Liver bypass significantly increases the transduction efficiency of recombinant adenoviral vectors in the lung, intestine, and kidney
32. Lack of high affinity fiber receptor activity explains the resistance of ciliated airway epithelia to adenovirus infection
33. Imaging and tissue biodistribution of 99mTc-labeled adenovirus knob (serotype 5)