A flow cell assay for evaluation of whole cell drug efflux kinetics: Analysis of paclitaxel efflux in CCRF-CEM leukemia cells overexpressing P-glycoprotein

Drug Metabolism and Disposition

Volumn 29, Issue 2, 2001, Pages 103-110

  Lin, James T ^a   Sharma, Rashmi ^a,c   Grady, James J ^b   Awasthi, Sanjay ^a,d  

^a University of Texas Medical Branch School of Medicine   (United States)

^b UNIVERSITY OF TEXAS MEDICAL BRANCH   (United States)

^c U S ENVIRONMENTAL PROTECTION AGENCY   (United States)

^d UNIVERSITY OF TEXAS AT ARLINGTON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GLYCOPROTEIN P; PACLITAXEL; VERAPAMIL;

ACUTE LEUKEMIA; ARTICLE; CELL ADHESION; CELL SUSPENSION; DRUG ACCUMULATION; DRUG TRANSPORT; FLOW CYTOMETRY; GENE OVEREXPRESSION; HUMAN; HUMAN CELL; LINEAR SYSTEM; MEMBRANE PERMEABILITY; MULTIDRUG RESISTANCE; PASSIVE TRANSPORT; PRIORITY JOURNAL; TEMPERATURE SENSITIVITY; TRANSPORT KINETICS;

ANTINEOPLASTIC AGENTS, PHYTOGENIC; BIOLOGICAL TRANSPORT, ACTIVE; DIFFUSION; DRUG RESISTANCE, MULTIPLE; FLOW CYTOMETRY; HUMANS; KINETICS; LEUKEMIA, MYELOID; P-GLYCOPROTEIN; PACLITAXEL; TEMPERATURE; TUMOR CELLS, CULTURED; VERAPAMIL;

EID: 0035154146     PISSN: 00909556     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (37)

1. Co-operative, competitive and non-competitive interactions between modulators of Pgp
2. Mechanisms of drug resistance
3. Altered surface membrane glycoproteins in vinca alkaloid-resistant human leukemic lymphoblasts
4. Characteristic of a human myeloid leukemia cell line highly resistant to Taxol
5. Cellular resistance to actinomycin D in Chinese hamster ovary cells in vitro: Cross resistance, radioautographic, and cytogenetic studies
6. Analysis of drug transport kinetics in multidrug-resistant cells using a novel coumarin-vinblastine compound
7. A role for paclitaxel in the combination chemotherapy of acute myeloblastic leukaemia: Preclinical cell culture studies
8. A model for computer simulation of P-glycoprotein and transmembrane ΔpH-mediated anthracycline transport in multidrug-resistant tumor cells
9. Potentiation of anticancer-drug cytotoxicity by multidrug resistance chemosensitizers involves alterations in membrane fluidity leading to increased membrane permeability
10. Resistance mechanisms in human sarcoma mutants derived by single-step exposure to paclitaxel (Taxol)
11. Transport of polypeptide ionophores into proteoliposomes reconstituted with rat liver P-glycoprotein
12. Functional reconstitution of P-glycoprotein reveals an apparent near stoichiometric drug transport to ATP hydrolysis
13. Analysis of the tangled relationships between P-glycoprotein-mediated multidrug resistance and the lipid phase of the cell membrane
14. The myocardia cell surface, its histochemistry, and the effect of sialic acid and calcium removal on its structure and cellular ionic exchange
15. Protein-carbohydrate interaction. II. Inhibition studies on the interaction of concanavalin A with polysaccharides
16. Is the multidrug transporter a flippase?
17. ATP-dependent transport of vinblastine in vesicles from human multidrug-resistant cells
18. Paclitaxel: Mechanisms of action and resistance
19. Reversal mechanism of multidrug resistance by verapamil: Direct binding of verapamil to Pgp on specific sites, and transport of verapamil outward across the plasma membrane of K562 ADM cells
20. Pgp expression at diagnosis may not be a primary mechanism of therapeutic failure in childhood rhabdomyosarcoma
21. Phase I study of Taxol in refractory acute myelogenous leukemias using a weekly schedule
22. Altered molecular properties of tubulin in a multidrug-resistant variant of Chinese hamster cells selected for resistance to vinca alkaloids
23. Transmembrane aromatic amino acid distribution in Pgp. A functional role in broad substrate specificity
24. Binding of an optically pure photoaffinity analogue of verapamil, LU-49888, to Pgp from multi-drug resistant human leukemic cell lines
25. Genetic and biochemical characterization of multidrug resistance
26. The role of the MDR protein in altered drug translocation across tumor cell membranes
27. Antimicrotubule agents
28. Functional expression of P-glycoprotein in secretory vesicles
29. Drug membrane interactions and the importance for drug transport, distribution, accumulation, efficacy and resistance
30. Reconstitution of drug transport by purified P-glycoprotein
31. Synthetic hydrophobic peptides are substrates for P-glycoprotein and stimulate drug transport
32. Functional reconstitution of drug transport and ATPase activity in proteoliposomes containing partially purified Pgp
33. Markedly altered membrane transport and intracellular binding of vincristine in multidrug-resistant Chinese hamster cells selected for resistance to vinca alkaloids
34. Kinetics of daunorubicin transport by Pgp of intact cancer cells
35. The role of drug-lipid interactions in the biological activity of modulators of multi-drug resistance
36. The relative importance of passive and P-glycoprotein mediated anthracycline efflux from multidrug-resistant cells
37. Structure and function of multidrug transporters