Protein unfolding during reversed-phase chromatography: I. Effect of surface properties and duration of adsorption

Biotechnology and Bioengineering

Volumn 76, Issue 3, 2001, Pages 224-232

  McNay, Jennifer L M ^a,b   Fernandez, Erik J ^a  

^a University of Virginia   (United States)

^b REGENERON PHARMACEUTICALS INC   (United States)

Author keywords

Adsorption; Hydrogen exchange; Nuclear magnetic resonance; Protein conformation; Reversed phase chromatography

Indexed keywords

ADSORPTION; CHROMATOGRAPHY; CONFORMATIONS; DEUTERIUM; HYDROGEN; HYDROPHOBICITY; MOLECULES; NUCLEAR MAGNETIC RESONANCE; PORE SIZE; SILICA;

REVERSE-PHASE CHROMATOGRAPHY;

PROTEINS;

APROTININ; DEUTERIUM; HYDROGEN;

ADSORPTION; ARTICLE; HYDROPHOBICITY; NUCLEAR MAGNETIC RESONANCE; POROSITY; PROTEIN FOLDING; PROTON TRANSPORT; REVERSED PHASE LIQUID CHROMATOGRAPHY; SURFACE PROPERTY;

ADSORPTION; ALKYLATION; APROTININ; CHROMATOGRAPHY; HYDROGEN BONDING; MAGNETIC RESONANCE SPECTROSCOPY; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN FOLDING;

BOVINAE;

EID: 0035152884     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10015     Document Type: Article

References (32)

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19. 1H NMR
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