Encapsulation of proteins by layer-by-layer adsorption of polyelectrolytes onto protein aggregates: Factors regulating the protein release

Biotechnology and Bioengineering

Volumn 76, Issue 3, 2001, Pages 207-213

  Balabushevitch, Nadejda G ^a   Sukhorukov, Gleb B ^a,c   Moroz, Natalia A ^a   Volodkin, Dmitry V ^b   Larionova, Natalia I ^a   Donath, Edwin ^a,b   Mohwald, Helmuth ^b  

^a MOSCOW STATE UNIVERSITY   (Russian Federation)

^b MAX PLANCK INSTITUTE OF COLLOIDS AND INTERFACES   (Germany)

^c SHUBNIKOV INSTITUTE OF CRYSTALLOGRAPHY   (Russian Federation)

Author keywords

Colloid; Microcapsules; Polyelectrolytes; Protein aggregates; Protein encapsulation; Protein release; Ultra thin organic films

Indexed keywords

ADSORPTION; AGGREGATES; ENCAPSULATION; MEMBRANES; MOLECULAR WEIGHT; POLYELECTROLYTES; SOLUBILITY;

POLYELECTROLYTE LAYERS;

PROTEINS;

CHYMOTRYPSIN; POLYELECTROLYTE;

ADSORPTION; ARTICLE; MEMBRANE; MEMBRANE PERMEABILITY; MOLECULAR STABILITY; MOLECULAR WEIGHT; PROTEIN AGGREGATION; PROTEIN SECRETION;

ADSORPTION; CAPSULES; CHYMOTRYPSIN; HYDROGEN-ION CONCENTRATION; SUBSTRATE SPECIFICITY; TRYPSIN INHIBITORS;

EID: 0035148750     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.1184     Document Type: Article

References (28)

1. Detection of light signals by microencapsulated cis-cinnamoyl chymotrypsin
2. Lactate dehydrogenase in interpolyelectrolyte complex. Function and stability
3. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
4. Fuzzy nanoassemblies: Toward layered polymeric multicomposites
5. Build up of ultrathin multilayer films by a self-assembly process: I. Consecutive adsorption of anionic and cationic bipolar amphiphiles
6. New composite films for biosensors: Layer-by-layer adsorbed films of polyelectrolytes, proteins or DNA
7. Novel hollow polymer shells: Fabrication, characterization and potential applications
8. Biochemistry and applications of aprotinin, the kallikrein inhibitor from bovine organs
9. Electroluminescence studies on self-assembled films of PPV and CdSe nanoparticles
10. Nonspecific catalyses by α-chymotrypsin and trypsin
11. Protein measurement with the Folin phenol reagent
12. Layer-by-layer architecture of concavalin A by means of electrostatic and biospecific interactions
13. Protein architecture: Assembly of ordered films by means of alternated adsorption of oppositely charged macromolecules
14. Fabrication of microporous thin films from polyelectrolyte multilayers
15. Ultrathin self-assembled polyelectrolyte membranes for pervaporation
16. Interaction of chymotrypsin with cinnamoylimidazole, substrate sensitive to light
17. Sequential actions of glucose oxidase and peroxidase in molecular films assembled by layer-by-layer adsorption
18. Sequential reaction and product separation on molecular films of glucoamylase and glucose oxidase assembled on an ultrafilters
19. Immobilization of proteins and cell fragments by use of a new method of microencapsulation
20. Immobilization of enzymes by multilayer microcapsules
21. The spectrophotometric determination of the operational normality of an α-chymotrypsin solution
22. Layer-by-layer self-assembly of polyelectrolytes onto colloidal particles
23. Step-wise polyelectrolyte assembly on particle surfaces B. A novel approach to colloid design
24. Multilayer films containing immobilized nucleic acids. Their structure and possibilities in biosensor applications
25. pH-controlled macromolecule encapsulation in and release from polyelectrolyte multilayer nanocapsules
26. Proton concentration profile in ultrathin polyelectrolyte films
27. Self-assembled second-order nonlinear optical multilayer azo polymer