RT-PCR-ELOSA tests on pooled sample units for the detection of virus Y in potato tubers

Journal of Virological Methods

Volumn 91, Issue 2, 2001, Pages 99-108

  Chandelier, A ^a   Dubois, N ^a   Baelen, F ^a   De Leener, F ^b   Warnon, S ^c   Remacle, J ^c   Lepoivre, P ^a  

^a UNIVERSITY OF LIÈGE   (Belgium)

^b Parc Crealys   (Belgium)

^c UNIVERSITY OF NAMUR   (Belgium)

Author keywords

Diagnosis; Enzyme linked immunosorbent assay (ELISA); Plant; Potato virus Y (PVY); Virus

Indexed keywords

COAT PROTEIN;

ARTICLE; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYME LINKED OLIGOSORBENT ASSAY; IMMUNOASSAY; NONHUMAN; POTATO; POTYVIRUS; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIRUS CHARACTERIZATION; VIRUS DETECTION; VIRUS GENE;

DNA PRIMERS; DNA, VIRAL; ENZYME-LINKED IMMUNOSORBENT ASSAY; PLANTS, TOXIC; POTYVIRUS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SOLANUM TUBEROSUM; TOBACCO; TRANSCRIPTION, GENETIC;

POTATO VIRUS Y; SOLANUM TUBEROSUM;

EID: 0035146694     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(00)00238-X     Document Type: Article

References (30)

1. Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses
2. Potato virus A detection by reverse transcription-polymerase chain reaction
3. Detection of potato virus Y in early-harvested potato tubers by cDNA hybridisation and three modifications of ELISA
4. Potato virus Y
5. Trends in France for the use of the ELISA method in routine seed-potato testing and problems of cross reactions between potato viruses A and Y
6. Quality-control programs for seedborne pathogens
7. Le test immuno-enzymatique (ELISA) et son application pour le diagnostic rapide des viroses de la pomme de terre
8. An improved purification procedure for preparing potyviruses and cytoplasmic inclusions from the same tissue
9. A PCR-microplate hybridization method for plant virus detection
10. Characterization and identification of potato viruses and viroids. Physical and chemical properties
11. Biological characterisation of various geographical isolates of potato virus Y inducing superficial necrosis in potato tubers
12. Detection of apple stem grooving virus in dormant apple trees with crude extracts as templates for the one-step RT-PCR
13. A simple, rapid method of nucleic acid extraction without tissue homogenisation for detecting viroids by hybridisation and RT-PCR
14. A method combining immunocapture and PCR amplification in a microtiter plate for the detection of plant viruses and subviral pathogens
15. Covalent immobilization of DNA onto polystyrene microwells: The molecules are only bound at the 5′ end
16. Nucleotide sequence of potato virus Y (N strain) genomic RNA
17. Detection of potato leafroll virus in dormant potato tubers by immunocapture and a fluorogenic 5′ nuclease RT-PCR assay
18. Comparison of three RT-PCR methods
19. Reverse-transcription polymerase chain reaction for the detection of viruses from plants and aphids
20. Detection of stylet-borne and circulative potato viruses in aphids by duplex reverse transcription polymerase chain reaction
21. Detection of potato leafroll virus in single aphids by the reverse transcription polymerase chain reaction and its potential epidemiological application
22. Factors affecting detection of PVY in dormant tubers by reverse transcription polymerase chain reaction and nucleic acid spot hybridization
23. Specific detection of potato virus A in dormant tubers by reverse transcription polymerase chain reaction
24. Use of citric acid for neutralizing polymerase chain reaction inhibition by chlorogenic acid in potato extracts
25. Two rapid microscale procedures for isolation of total RNA from leaves rich in polyphenols and polysaccharides: Application for sensitive detection of grapevine viroids
26. Yield reduction in relation to virus infection
27. Detection of potato viruses Y and A in tubers by enzyme-linked immunosorbent assay after natural and artificial break of dormancy
28. Detection of the potato tuber necrotic ringspot strain of potato virus Y (PVYNTN) by reverse transcription and immunocapture polymerase chain reaction
29. DNA probe hybridisation in microwells using a new bioluminescent system for the detection of PCR-amplified HIV-1 proviral DNA