The C-terminal residues of poliovirus proteinase 2Apro are critical for viral RNA replication but not for cis- or trans-proteolytic cleavage

Journal of General Virology

Volumn 82, Issue 2, 2001, Pages 397-408

  Li, X ^a   Lu, H H ^a   Mueller, S ^a   Wimmer, E ^a  

^a STONY BROOK UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; CELL PROTEIN; PROLINE; PROTEINASE; VIRUS ENZYME; VIRUS PROTEIN; VIRUS RNA;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CHIMERA; CIS TRANS ISOMERISM; CONTROLLED STUDY; ENTEROVIRUS; GENE DELETION; GENE SEQUENCE; GENETIC CODE; HUMAN RHINOVIRUS; MUTATION; NONHUMAN; POLIOMYELITIS VIRUS; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN MOTIF; PROTEIN PROCESSING; RNA REPLICATION; VIRUS INFECTION; VIRUS PARTICLE; VIRUS REPLICATION;

ENTEROVIRUS; HUMAN POLIOVIRUS 1; HUMAN RHINOVIRUS SP.; POLIOVIRUS; RNA VIRUSES;

EID: 0035146234     PISSN: 00221317     EISSN: None     Source Type: Journal    
DOI: 10.1099/0022-1317-82-2-397     Document Type: Article

References (78)

1. Paradoxes of the replication of picornaviral genomes
2. pro expression in mammalian cells: Effects on vaccinia virus
3. Polioviruses containing picornavirus type 1 and/or type 2 internal ribosomal entry site elements: Genetic hybrids and the expression of a foreign gene
4. Coinfection with recombinant vaccinia viruses expressing poliovirus P1 and P3 proteins results in polyprotein processing and formation of empty capsid structures
5. Complementation of a poliovirus defective genome by a recombinant vaccinia virus which provides poliovirus P1 capsid precursor in trans
6. Encapsidation and serial passage of a poliovirus replicon which expresses an inactive 2A proteinase
7. Enteroviral protease 2A cleaves dystrophin: Evidence of cytoskeletal disruption in an acquired cardiomyopathy
8. Encapsidation studies of poliovirus subgenomic replicons
9. pro expression inhibits growth of yeast cells
10. pro: Effects on cellular and viral gene expression
11. Viral cysteine proteases are homologous to the trypsin-like family of serine proteases: Structural and functional implications
12. Genetic complementation among poliovirus mutants derived from an infectious cDNA clone
13. The predominant elF4G-specific cleavage activity in poliovirus-infected HeLa cells is distinct from 2A protease
14. Direct cleavage of elF4G by poliovirus 2A protease is inefficient in vitro
15. RNA secondary structure and binding sites for gag gene products in the 5′ packaging signal of human immunodeficiency virus type 1
16. Cytoplasmic expression system based on constitutive synthesis of bacteriophage T7 RNA polymerase in mammalian cells
17. Poliovirus 2A protease induces apoptotic cell death
18. Internal ribosomal entry site substitution eliminates neurovirulence in intergeneric poliovirus recombinants
19. Genetics, pathogenesis, and evolution of picornaviruses
20. The elF4G-eIF4E complex is the target for direct cleavage by the rhinovirus 2A proteinase
21. Translational enhancement of the poliovirus 5′ noncoding region mediated by virus-encoded polypeptide 2A
22. Hepatitis A viruses with deletions in the 2A gene are infectious in cultured cells and marmosets
23. Characterization of poliovirus 2A proteinase by mutational analysis: Residues required for autocatalytic activity are essential for induction of cleavage of eukaryotic initiation factor 4F polypeptide p220
24. Determinants of substrate recognition by poliovirus 2A proteinase
25. Cleavage of poly(A)-binding protein by enterovirus proteases concurrent with inhibition of translation in vitro
26. Cleavage of poly(A)-binding protein by coxsackievirus 2A protease in vitro and in vivo: Another mechanism for host protein synthesis shutoff?
27. The genome-linked protein of picornaviruses. VII. Genetic mapping of poliovirus VPg by protein and RNA sequence studies
28. Primary structure, gene organization and polypeptide expression of poliovirus RNA
29. Proteolytically active 2A proteinase of human rhinovirus 2 is toxic for Saccharomyces cerevisiae but does not cleave the homologues of elF-4 gamma in vivo or in vitro
30. Poliovirus proteinase 2A induces cleavage of eukaryotic initiation factor 4F polypeptide p220
31. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
32. Mapping the cleavage site in protein synthesis initiation factor elF-4 gamma of the 2A proteases from human coxsackievirus and rhinovirus
33. Picornavirus protein processing -enzymes, substrates, and genetic regulation
34. pro-mediated, alternative cleavage of polypeptide 3CD by in vitro mutagenesis
35. A protein covalently linked to poliovirus genome RNA
36. Purification of two picornaviral 2A proteinases: Interaction with eIF-4γ and influence on in vitro translation
37. Poliovirus chimeras replicating under the translational control of genetic elements of hepatitis C virus reveal unusual properties of the internal ribosomal entry site of hepatitis C virus
38. Construction and genetic analysis of dicistronic polioviruses containing open reading frames for epitopes of human immunodeficiency virus type 1 gp120
39. pro proteins: Separation of proteinase from translation and replication functions
40. The 5′ noncoding region and virulence of poliovirus vaccine strains
41. The bovine leukemia virus encapsidation signal is discontinuous and extends into the 5′ end of the gag gene
42. The amino-terminal nine amino acid sequence of poliovirus capsid VP4 protein is sufficient to confer N-myristoylation and targeting to detergent-insoluble membranes
43. Protein 2A is not required for Theiler's virus replication
44. Cell-free, de novo synthesis of poliovirus
45. Cardioviral internal ribosomal entry site is functional in a genetically engineered dicistronic poliovirus
46. Inhibition of proteolytic activity of poliovirus and rhinovirus 2A proteinases by elastase-specific inhibitors
47. pro in RNA replication
48. Expression of foreign proteins by poliovirus polyprotein fusion: Analysis of genetic stability reveals rapid deletions and formation of cardiovirus-like open reading frames
49. Picornaviruses as tools of antigen delivery
50. Function of minor polypeptides in foot-and-mouth disease virus and poliovirus
51. Functional coupling between replication and packaging of poliovirus replicon RNA
52. pro mutant correlates with phosphorylation of the alpha subunit of eucaryotic initiation factor 2
53. Sequence alignments of picornavirus capsid proteins
54. The structure of the 2A proteinase from a common cold virus: A proteinase responsible for the shut-off of host-cell protein synthesis
55. Polioviruses: Molecular biology
56. Demonstration of the specificity of poliovirus encapsidation using a novel replicon which encodes enzymatically active firefly luciferase
57. Virus taxonomy at the XIth International Congress of Virology, Sydney, Australia, 1999
58. Characterization of poliovirus clones containing lethal and nonlethal mutations in the genome-linked protein VPg
59. Coding changes in the poliovirus protease 2A compensate for 5′NCR domain V disruptions in a cell-specific manner
60. Picornaviridae and their replication
61. The structure of picornaviral proteinases
62. Human rhinovirus 2: Complete nucleotide sequence and proteolytic processing signals in the capsid protein region
63. Substrate requirements of a human rhinoviral 2A proteinase
64. Others Polypeptide 2A of human rhinovirus type 2: Identification as a protease and characterization by mutational analysis
65. The 2A proteinase of human rhinovirus is a zinc containing enzyme
66. A second virus-encoded proteinase involved in proteolytic processing of poliovirus polyprotein
67. Synthesis of infectious poliovirus RNA by purified T7 RNA polymerase
68. pro mutant unable to cleave 3CD shows inefficient viral protein synthesis and transactivation defects
69. pro on translation and transcription
70. pro-binding peptides
71. Evidence for specificity in the encapsidation of Sindbis virus RNAs
72. Transgenic expression of replication-restricted enteroviral genomes in heart muscle induces defective excitation-contraction coupling and dilated cardiomyopathy
73. Genetics of poliovirus
74. Pro cleaves the TATA-binding protein but does not inhibit host cell RNA polymerase II transcription in vitro
75. Identification of essential amino acid residues in the functional activity of poliovirus 2A protease
76. Defective RNA replication by poliovirus mutants deficient in 2A protease cleavage activity