Scaling up the ligase chain reaction-based approach to gene synthesis

BioTechniques

Volumn 30, Issue 2, 2001, Pages 249-252

  Chalmers, F M ^a   Curnow, K M ^a  

^a BAKER IDI HEART AND DIABETES INSTITUTE   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

DNA POLYMERASE; LIGASE; OLIGONUCLEOTIDE; RECOMBINANT PROTEIN; SYNTHETIC DNA;

AMINO ACID SEQUENCE; ARTICLE; BASE PAIRING; DNA PURIFICATION; DNA SEQUENCE; GENE SEQUENCE; HIGH TEMPERATURE; HUMAN; LIGASE CHAIN REACTION; MOLECULAR CLONING; PHOSPHORYLATION; POLYMERASE CHAIN REACTION; RNA SEQUENCE; SEQUENCE ANALYSIS;

EID: 0035135741     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/01302bm02     Document Type: Article

References (8)

1. Gene synthesis by a LCR-based approach: High-level production of leptin-L54 using synthetic gene in Escherichia coli
2. Gene synthesis, high-level expression, and mutagenesis of Trio baciltus ferrooxidans rusticyanin: His 85 is a ligand to the blue copper center
3. PCR-based gene synthesis and protein NMR spectroscopy
4. PCR fidelity of Pfu DNA polymerase and other thermostable DNA polymerases
5. Baculovirus expression of human basic fibroblast growth factor from a synthetic gene: Role of the Kozak consensus and comparison with bacterial expression
6. Expression and codon usage optimization of the erythroid-specific transcription factor cGATA-1 in baculoviral and bacterial systems
7. Recursive PCR: A novel technique for total gene synthesis
8. Rapid evolution of a protein in vitro by DNA shuffling