Method for the immunological detection of silver-stained proteins on nitrocellulose membranes

BioTechniques

Volumn 30, Issue 2, 2001, Pages 266-272

  Scharfke M ^a   Baumeister, H ^a   Meyerhof, W ^a  

^a GERMAN INSTITUTE OF HUMAN NUTRITION   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AMIDO BLACK; PROTEIN; PYROXYLIN; SILVER;

ACCURACY; ARTICLE; CELL STRAIN HEPG2; GEL ELECTROPHORESIS; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; MASS SPECTROMETRY; OXIDATION; PEPTIDE MAPPING; PROTEIN ANALYSIS; PROTEIN IMMOBILIZATION; REACTION ANALYSIS; RELIABILITY; SILVER STAINING; TWO DIMENSIONAL GEL ELECTROPHORESIS; WESTERN BLOTTING;

EID: 0035134168     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/01302bm06     Document Type: Article

References (12)

1. Improved silver staining of plant proteins
2. Two-dimensional polyacrylamide gel electrophoresis with immobilized pH gradients in the first dimension (IPG-Dalt): The state of the art and the controversy of vertical versus horizontal systems
3. Tissue-specific in vitro transcription from the mouse albumin promoter
4. Gel protein stains: Silver stain
5. Sensitive colloidal metal (gold or silver) staining of protein blots on nitrocellulose membranes
6. Reversible staining and peptide mapping of proteins transferred to nitrocellulose after separation by sodium dodecylsulfate-polyacrylamide gel electrophoresis
7. Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels
8. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications
9. Analysis of blood plasma proteins in patients with Alzheimer's disease by two-dimesional electrophoresis, sequence homology and immunodetection
10. Studies and critique of amido black 10B, coomassie blue R, and fast green FCF as stains for proteins after polyacrylamide gel electrophoresis
11. Matrix assisted laser desorption/ionization-time of flight-mass spectrometry analysis of proteins detected by anti-phosphotyrosine antibody on two-dimensional-gels of fibroblast cell lysates after tumor necrosis factor-α stimulation
12. A new method to assign immuno-detected spots in the complex two-dimensional electrophoresis pattern