A simple contrast enhancement by potassium permanganate oxidation for Lowicryl K4M ultrathin sections prepared by high pressure freezing/freeze substitution

Journal of Microscopy

Volumn 201, Issue 1, 2001, Pages 77-83

  Sawaguchi, A ^a   Ide, S ^a   Goto, Y ^a   Kawano, J I ^a   Oinuma, T ^a   Suganuma, T ^a  

^a UNIVERSITY OF MIYAZAKI   (Japan)

Author keywords

Colloidal gold labelling; Contrast enhancement; High pressure freezing; Lectin cytochemistry; Lowicryl K4M; Permanganate oxidation

Indexed keywords

BIOCHEMISTRY; CELL MEMBRANES; COLLOIDS; CYTOLOGY; FREEZING; GOLD NANOPARTICLES; LEAD COMPOUNDS; OXIDATION; POTASH; URANIUM COMPOUNDS;

COLLOIDAL GOLD; COLLOIDAL GOLD LABELING; CONTRAST ENHANCEMENT; FREEZE SUBSTITUTION; GOLD LABELING; HIGH-PRESSURE FREEZING; LECTIN CYTOCHEMISTRY; LOWICRYL K4M; PERMANGANATE OXIDATION; ULTRA-THIN;

MANGANESE COMPOUNDS;

PERMANGANATE POTASSIUM;

ACCURACY; ANIMAL TISSUE; ARTICLE; CELL MEMBRANE; CHEMICAL LABELING; CONTRAST ENHANCEMENT; CONTROLLED STUDY; CYTOCHEMISTRY; FREEZE DRYING; HISTOCHEMISTRY; IMMUNOCYTOCHEMISTRY; MALE; NONHUMAN; PRIORITY JOURNAL; RAT; STAINING; TISSUE SECTION;

ANIMALIA;

EID: 0035130868     PISSN: 00222720     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2818.2001.00787.x     Document Type: Article

References (17)

1. High resolution light and electron microscopic localization of tubulin with the IGS (immuno gold staining) method
2. Advances in ultrarapid freezing for the preservation of cellular ultrastructure
3. Principles and techniques of electron microscopy
4. Immunocytochemical localisation of actin and profilin in the generative cell of angiosperm pollen: TEM studies on highpressure frozen and freeze-substituted Ledebouria socialis Roth (Hyacinthaceae)
5. Alternative staining methods for Lowicryl sections
6. The potential of cryofixation and freeze substitution: Observations and theoretical considerations
7. Contrast-enhancement for the image of human immunodeficiency virus from ultrathin section by immuno electron microscopy
8. The use of potassium permanganate as an electron-dense stain for sections of tissue embedded in epoxy resin
9. High-pressure freezing for immunocytochemistry
10. Snap-freezing under high pressure: A new fixation technique for freeze-etching
11. Postembedding labeling on Lowicryl K4M tissue sections: Detection and modification of cellular components
12. Contrasting of Lowicryl K4M thin sections
13. Reappraisal of potassium permanganate oxidation applied to Lowicryl K4M embedded tissues processed by high pressure freezing/freeze substitution, with special reference to differential staining of the zymogen granules of rat gastric chief cells
14. A new method of preparing gold probes for multiple-labeling cytochemistry
15. Buffered potassium permanganate-uranyl acetate-lead citrate staining sequence for ultrathin sections
16. High pressure freezing technique and application to histochemistry
17. 2)