Monitoring of the refolding process for immobilized firefly luciferase with a biosensor based on surface plasmon resonance

Journal of Biochemistry

Volumn 129, Issue 1, 2001, Pages 1-4

  Zako, Tamotsu ^a   Harada, Kenkichi ^a   Mannen, Teruhisa ^a   Yamaguchi, Satoshi ^a   Kitayama, Atsushi ^a   Ueda, Hiroshi ^a   Nagamune, Teruyuki ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

Firefly luciferase; Immobilization; Refolding; SPR sensor

Indexed keywords

CARBOXYMETHYLDEXTRAN; LUCIFERASE;

ANIMAL CELL; ARTICLE; BIOSENSOR; CIRCULAR DICHROISM; CONFORMATIONAL TRANSITION; ENZYME ACTIVITY; FLY; IMMOBILIZED CELL; MONITORING; NONHUMAN; PROTEIN DNA INTERACTION; PROTEIN FOLDING; REFRACTION INDEX; SURFACE PLASMON RESONANCE;

ANIMALS; BEETLES; BIOSENSING TECHNIQUES; LUCIFERASES; PROTEIN CONFORMATION; PROTEIN FOLDING; SURFACE PLASMON RESONANCE;

ANIMALIA;

EID: 0035125496     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a002818     Document Type: Article

References (9)

1. The application of circular dichroism to studies of protein folding and unfolding
2. The radius of gyration of an apomyoglobin folding intermediate
3. Kinetic and circular dichroism studies of enzymes adsorbed on ultrafine silica particles
4. BIAcore for macromolecular interaction
5. Detection of conformational changes in an immobilized protein using surface plasmon resonance
6. Refolding of firefly luciferase immobilized on agarose beads
7. Equilibrium intermediates in the reversible unfolding of firefly (Photinus pyralis) luciferase
8. Folding of firefly (Photinus pyralis) luciferase: Aggregation and reactivation of unfolding intermediates