Cell size as a tool to predict the production of recombinant protein by the insect-cell baculovirus expression system

Biotechnology Letters

Volumn 23, Issue 5, 2001, Pages 359-364

  Palomares, Laura A ^a   Pedroza, Juan Carlos ^b   Ramírez, Octavio T ^a  

^a INSTITUTO DE BIOTECNOLOGÍA   (Mexico)

^b UNIVERSIDAD AUTÓNOMA DEL ESTADO DE MORELOS   (Mexico)

Author keywords

Baculovirus; Culture monitoring; Insect cell size; Metabolism; Recombinant protein

Indexed keywords

RECOMBINANT PROTEIN;

ANIMAL CELL; ARTICLE; BACULOVIRUS; CELL SIZE; IMMUNOBLOTTING; LEPIDOPTERA; NONHUMAN; PREDICTION; PROTEIN SYNTHESIS;

ANIMALIA; BOVINAE; INSECTA; LEPIDOPTERA; PROKARYOTA; ROTAVIRUS; UNIDENTIFIED BACULOVIRUS;

EID: 0035119844     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1005688417525     Document Type: Article

References (20)

1. Insect cell line dependent gene expression of recombinant tumor necrosis factor-β
2. The growth and nutrient utilization of insect cell line Spodoptera frugiperda Sf9 in batch and continuous culture
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4. Replacement of vertebrate serum with lipids and other factors in the culture of invertebrate cells, tissue, parasites and pathogens
5. Established insect cell line from the cabbage looper
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7. Nutrient optimization for high density biological production applications
8. Online monitoring of respiration in recombinant-baculovirus infected and uninfected insect cell bioreactor cultures
9. A method to determine the optimal time to infect insect cells with the baculovirus expression system
10. An insight into insect cell metabolism through selective nutrient manipulation
11. Comparative characterization of cell death between Sf9 insect cells and hybridoma cultures
12. Glutamine is not an essential amino acid for Sf-9 insect cells
13. Introduction of a metabolic switch in insect cells by substrate-limited fed batch cultures
14. The effect of dissolved oxygen tension and the utility of oxygen uptake rate in insect cell culture
15. Evidence of Pluronic F-68 direct interaction with insect cells: Impact on shear protection, recombinant protein and baculovirus production
16. Cell cycle and growth phase-dependant variations in size distributions, antibody productivity, and oxygen demand in hybridoma cultures
17. Osmolarity effects on observed cell size after baculovirus are avoided using growth medium for sample dilution
18. DNA distribution and respiratory activity of Spodoptera frugiperda populations infected with wild-type and recombinant Autographa californica nuclear polyhedrosis virus
19. Effect of elevated oxygen and glutamine level on foreign protein production at high cell densities using the insect cell-baculovirus expression system
20. Expression of epoxide hydrolase in insect cells: A focus on the infected cell