Analysis of the non-deletion α-thalassemia mutations by PCR temperature gradient gel electrophoresis

Chinese Journal of Medical Genetics

Volumn 18, Issue 1, 2001, Pages 51-55

  Yongzhong, Z ^a   Xiangmin, X ^a   Yang, Y ^a  

^a SOUTHERN MEDICAL UNIVERSITY   (China)

Author keywords

globin gene; thalassemia; Temperature gradient gel electrophoresis

Indexed keywords

ALPHA 2 GLOBULIN;

ALPHA THALASSEMIA; ARTICLE; CHINESE; DNA POLYMORPHISM; DNA SEQUENCE; GEL ELECTROPHORESIS; GENETIC SCREENING; HUMAN; PATHOGENESIS; POINT MUTATION; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS;

ALPHA-THALASSEMIA; ELECTROPHORESIS; GLOBINS; HUMANS; POINT MUTATION; POLYMERASE CHAIN REACTION; POLYMORPHISM, GENETIC; TEMPERATURE;

EID: 0035116692     PISSN: 10039406     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (17)

1. A review of the molecular genetics of the human α-globin gene cluster
2. Different forms of Hb H disease in the Chinese
3. Molecular defects in Hb H hydrops fetalis
4. A supplement to the hemoglobin and thalassemia syllabi
5. Molecular basis of non-deletion α-thalassemia
6. The types and distribution of α-thalassemia-2 in China
7. DNA fragment differing by single base-pair substitutions are separated in denaturing gradient gels: Correspondence with melting theory
8. Detecting single base substitutions, mismatches and bulges in DNA by temperature gradient gel electrophoresis and related methods
9. The thalassemias
10. Locus assignment of human α-globin mutations by selective amplification and direct sequencing
11. Rapid detection of point mutation and polymorphism of the α-globin genes by DGGE and SSCP
12. 2, EDTA, storage time and temperature on DNA yield and quality
13. PCR-SSCP: A method for detection of mutations
14. Rapid detection of non-deletion alpha-thalassemia mutations by PCR-LIS-SSCP
15. Temperature gradient gel electrophoresis: Thermodynamic analysis of nucleic acids and protein in puried form and in cellular extracts
16. Thermal denaturation of double-stranded nucleic acids: Prediction of temperatures critical for gradient gel electrophoresis and polymerase chain reaction
17. Temperature-gradient gel electrophoresis as a screening tool for polymorphism in multigene families