Integrated RT-PCR/nested PCR diagnosis for differentiating between subgroups of plum pox virus

Journal of Virological Methods

Volumn 92, Issue 2, 2001, Pages 165-175

  Szemes, Marianna ^a   Kálmán, Miklós ^a   Myrta, Arben ^c   Boscia, Donato ^d   Németh, Mária ^b   Kölber, Mária ^b   Dorgai, László ^a  

^a Institute for Biotechnology Szeged   (Hungary)

^b CENTRAL VETERINARY INSTITUTE   (Hungary)

^c ISTITUTO AGRONOMICO MEDITERRANEO   (Italy)

^d CNR   (Italy)

Author keywords

Differential diagnosis; Nested PCR; PCR; Plum pox virus (PPV)

Indexed keywords

COAT PROTEIN; COMPLEMENTARY DNA; OLIGONUCLEOTIDE; PRIMER DNA;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; CROSS REACTION; MOSAIC VIRUS; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEROLOGY; STRAIN DIFFERENCE; VALIDATION PROCESS; VIRUS DETECTION; VIRUS GENOME; VIRUS STRAIN; VIRUS TYPING;

AMINO ACID SEQUENCE; CAPSID; CAPSID PROTEINS; DNA PRIMERS; PLANT DISEASES; PLUM POX VIRUS; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; SEROTYPING;

PLUM POX VIRUS; POX VIRUS; PRUNUS DOMESTICA;

EID: 0035113866     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(00)00284-6     Document Type: Article

References (39)

1. First findings of plum pox virus in walnut trees
2. Production and characterization of a monoclonal antibody specific to the M serotype of plum pox potyvirus
3. Corrélation entretrois techniques permettant de différencier les isolates du plum pox potyvirus
4. Detection of plum pox potyvirus using monoclonal antibodies to structural and non-structural proteins
5. Analysis of plum pox virus variability and development of strain-specific PCR assays
6. Comparison of monoclonal antibodies and polymerase chain reaction assays for typing of isolates belonging to the D and M serotypes of plum pox potyvirus
7. Characterization of plum pox virus coatprotein epitopes using fusion proteins expressed in E. coli
8. Plum pox virus in sweet cherry
9. Synthetic peptides in diagnosis of plum pox potyvirus
10. A comprehensive set of sequence analysis programs for the VAX
11. Plum pox disease of stone fruit in Egypt
12. Plum pox: Advances in research on the disease and its casual agent, and possible means of control
13. Accurate identification of plum pox potyvirus and its differentiation from Asian prunus latent potyvirus in Prunus germplasms
14. A broad-spectrum PCR assay combined with RFLP analysis for detection and differentiation of plum pox virus isolates
15. Plum pox virus in cherry
16. Plum pox virus on sour cherry in Moldova
17. Differenciation biologique et serologique du souches du virus de la sharka
18. A simple and rapid method for processing tissue infected with plum pox potyvirus with specific 3′ non-coding region RT-PCR assays
19. Biotechnological aspects of plum pox virus
20. Detection and serotyping of Mediterranean plum pox virus isolates by means of strain-specific monoclonal antibodies
21. Production of a monoclonal antibody specific to the El Amar strain of Plum Pox Virus
22. Properties of two monoclonal antibodies specific to the cherry strain of plum pox virus
23. Sour cherry strain of plum pox potyvirus (PPV): Molecular and serological evidence for a new subgroup of PPV strains
24. History and importance of plum pox in stone-fruit production
25. Simultaneous detection and typing of plum pox potyvirus (PPV) isolates by heminested-PCR and PCR-ELISA
26. Comparative sequence analysis of four complete primary structures of plum pox virus strains
27. Plum pox potyvirus strains: An overview
28. Specific detection of D- and M-isolates of plum pox virus by immunoenzymatic determination of PCR products
29. Hungarian plum pox virus isolates represent different serotypes
30. A hazai szilvahimlo virus izolátumok genetikai polimorfizm usának bizonýtása RT-PCR módszerrel
31. Plum Pox situation in Europe
32. The N and C termini of the coat proteins of potyviruses are surface located and the N terminus contains the major virus specific epitopes
33. Plum pox virus
34. Improved detection of Prunus necrotic ringspot virus by the polymerase chain reaction
35. Plum pox virus on stone fruits from India. A new record
36. Detection of plum pox virus by ELISA in Prunus spp. at the different stages of their development
37. A polymerase chain reaction assay adapted to plum pox potyvirus detection
38. Nucleotide sequence of the 3′-terminal region of the RNA of the E1 Amar strain of plum pox potyvirus