Time-resolved study of the inner space of lactose permease

Biophysical Journal

Volumn 80, Issue 3, 2001, Pages 1498-1506

  Nachliel, E ^a   Pollak, N ^a   Huppert, D ^a   Gutman, M ^a  

^a TEL AVIV UNIVERSITY   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

LACTOSE PERMEASE;

ARTICLE; CATALYSIS; DISSOCIATION CONSTANT; ENZYME ANALYSIS; ENZYME STRUCTURE; ESCHERICHIA COLI; HYDROPHOBICITY; IONIC STRENGTH; NONHUMAN; PHOSPHOLIPID MEMBRANE; PROTEIN PROTEIN INTERACTION; RECEPTOR BINDING; STOICHIOMETRY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PHOCIDAE;

EID: 0035112524     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(01)76122-X     Document Type: Article

References (17)

1. Agmon, N. 1999. Proton solvation and proton mobility. Israel J. Chem. 39:493-502.
2. Agmon, N., E. Pines, and D. Huppert. 1988. Geminate recombination in proton-transfer reaction. II. Comparison of diffusional and kinetic schemes. J. Chem. Phys. 88:5631-5638.
3. Agmon, N., and A. Szabo. 1990. Theory of reversible diffusion-influenced reaction. J. Chem. Phys. 92:5270-5284.
4. Arakawa, T. T. S. 1982. Stabilization of protein structure by sugars. Biochemistry. 21:6536-6544.
5. Clement, N. R., and J. M. Gould. 1981. Pyranine as a probe of internal aqueous proton concentration in phospholipid vesicles. Biochemistry. 20:1534-1538.
6. Forster Th., and A. Volkers. 1975. Kinetics of proton transfer reactions involving hydroxypyrene-trisulfonate in aqueous solution by nanosecond laser absorption spectroscopy. Chem. Phys. Lett. 34:1-5.
7. Frillingos, S., M. Sahin-Toth, J. Wu, and H. R. Kaback. 1998. Cys-scanning mutagenesis: a novel approach to structure-function relationship in polytopic membrane proteins. FASEB J. 12:1281-1299.
8. Gutman, M., D. Huppert, and E. Nachliel. 1982. Kinetic studies of proton transfer in the microenvironment of a binding site. Eur. J. Biochem. 121:637-642.
9. Gutman, M., and E. Nachliel. 1990. The dynamic aspects of proton transfer processes. Biochim. Biophys. Acta. 1015:391-414.
10. Gutman, M., and E. Nachliel. 1993. Study of the semi ordered water in an active site by time resolved measurement of a single diffusing proton. Solid State Ionics. 61:229-234.
11. Gutman, M., and E. Nachliel. 1997. Time-resolved dynamics of proton transfer in proteinous systems. Annu. Rev. Phys. Chem. 48:329-356.
12. Gutman, M., E. Nachliel, and S. Kiryati. 1992a. Dynamic studies of proton diffusion in mesoscopic heterogeneous matrix, the interbilayer space between phospholipids membranes. Biophys. J. 63:281-290.
13. Gutman, M., Y. Tsfadia, A. Masad, and E. Nachliel. 1992b. Quantitation of physical-chemical properties of the aqueous phase inside the PhoE ionic channel. Biochim. Biophys. Acta. 1109:141-148.
14. Huppert, D., E. Kolodney, M. Gutman, and E. Nachliel. 1982. Effect of water activity on the rate of proton dissociation. J. Am. Chem. Soc. 104:6949-6953.
15. Huppert, D., E. Pines, and N. Agmon. 1990. Long-time behavior of reversible geminate recombination reactions. J. Otc. Soc. Am. 7:1545-1550.
16. Jessen-Marshall, A. E., N. J. Parker, and R. J. Brooker. 1997. Suppressor analysis of mutations in the loop 2-3 motif of lactose permease: Evidence that glycine-64 is an important residue for conformational changes. J. Bacterial. 179:2616-2622.
17. Johnson, J. L., and R. J. Brooker. 1999. A K319N/E325Q double mutant of the lactose permease cotransports H+ with lactose. Implications for a proposed mechanism of H+/lactose symport. J. Biol. Chem. 274:4074-4081.