Molecular cloning and expression in yeast of 2,3-oxidosqualene-triterpenoid cyclases from Arabidopsis thaliana

Plant Molecular Biology

Volumn 45, Issue 1, 2001, Pages 75-92

  Husselstein Muller, Tania ^a,b   Schaller, Hubert ^b   Benveniste, Pierre ^b  

^a UNIVERSITY OF FREIBURG   (Germany)

^b INST DE BIOL MOLEC DES PLANTES   (France)

Author keywords

and amyrin; 2,3 oxidosqualene; Arabidopsis; Intron; Lupeol; Multifunctional triterpene synthases

Indexed keywords

CATALYSIS; CHEMICAL STRUCTURE; COMPLEMENTARY DNA; CYCLOARTENOL SYNTHASE; EXON; INTRON; LANOSTEROL SYNTHASE; LUPEOL; MOLECULAR CLONING; RECOMBINANT PROTEIN; TERPENOID; TRITERPENOID;

AMINO ACID SEQUENCE; ARABIDOPSIS; CLONING, MOLECULAR; DNA, COMPLEMENTARY; DNA, RECOMBINANT; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENES, PLANT; INTRAMOLECULAR TRANSFERASES; MOLECULAR SEQUENCE DATA; OLEANOLIC ACID; PHYLOGENY; PLASMIDS; SACCHAROMYCES CEREVISIAE; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; TRITERPENES;

ARABIDOPSIS; ARABIDOPSIS THALIANA; EMBRYOPHYTA; EUKARYOTA;

EID: 0035111648     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1006476123930     Document Type: Article

References (44)

1. Active site mapping of affinity-labeled rat oxidosqualene cyclase
2. Molecular cloning, characterization, and functional expression of rat oxidosqualene cyclase cDNA
3. Purification of squalene-2,3-epoxide cyclases from cell suspension cultures of Rabdosia japonica Hara
4. Enzymatic cyclization of squalene and oxidosqualene to sterols and triterpenes
5. Sterol and triterpene synthesis in the developing and germinating pea seeds
6. Sterol biosynthesis
7. Nucleotide and deduced amino acid sequences of the oxidosqualene cyclase from Candida albicans
8. Cloning and functional expression in yeast of a cDNA coding for an obtusifoliol 14α-demethylase (CYP51) in wheat
9. Isolation of an Arabidopsis thaliana gene encoding cycloartenol synthase by functional expression in a yeast mutant lacking lanosterol synthase by the use of a chromatographic screen
10. Molecular cloning, characterization and overexpression of ERG7, the Saccharomyces cerevisiae gene encoding lanosterol synthase
11. Molecular cloning of a Schizosaccharomyces pombe cDNA encoding lanosterol synthase and investigation of conserved tryptophan residues
12. Methodology for the preparation of pure recombinant S. cerevisiae lanosterol synthase using a baculovirus expression system. Evidence that oxirane cleavage and A-ring formation are concerted in the biosynthesis of lanosterol from 2,3-oxidosqualene
13. Studies on the substrate binding segments and catalytic action of lanosterol synthase. Affinity labeling with carbocations derived from mechanism-based analogs of 2,3-oxidosqualene and site-directed mutagenesis probes
14. Triterpenoids
15. A plant DNA minipreparation: Version II
16. Evolution of genes and taxa: A primer
17. 7-sterol-C-5-desaturase by functional complementation of a defective yeast mutant
18. Analysis of pre-mRNA processing in transfected plant protoplasts
19. Plant intron sequences: Evidence for distinct groups of introns
20. Effect of gypsogenin 3,O-glucuronide pretreatment of Gypsophila paniculata and Saponaria officinalis cell suspension cultures on the activities of microsomal 2,3-oxidosqualene cycloartenol and amyrin cyclases
21. Cloning and characterization of the Arabidopsis thaliana lupeol synthase gene
22. Isolation and characterization of the gene encoding rat glucose-dependent insulinotropic peptide
23. Transformation of Saccharomyces cerevisiae with a cDNA encoding a sterol C-methyltransferase from Arabidopsis thaliana results in the synthesis of 24-ethyl sterols
24. Δ7-sterol-C5-desaturase: Molecular characterization and functional expression of wild type and mutant alleles
25. 4 response pathway in Arabidopsis, encodes a member of the rat family of protein kinase
26. β-amyrin synthase. Cloning of oxidosqualene cyclase that catalyzes the formation of the most popular triterpene among higher plants
27. Chimeric triterpene synthase. A possible model for multifunctional triterpene synthase
28. Triterpenoids from Euphorbia maculata
29. Preformed antimicrobial compounds and plant defense against fungal attack
30. Tree view: An application to display phylogenetic trees on personal computers
31. Compromised disease resistance in saponindeficient plants
32. A specific amino acid repeat in squalene and oxidosqualene cyclases
33. Sequence of the Candida albicans erg7 gene
34. An example of intron junctional sliding in the gene families encoding squalene monooxygenase homologues in Arabidopsis thaliana and Brassica napus
35. High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier
36. 3]acetate
37. Isolation and characterization of the gene encoding 2,3-oxidosqualene-lanosterol cyclase from Saccharomyces cerevisiae
38. Two branches of the lupeol synthase gene in the molecular evolution of plant oxidosqualene cyclases
39. Sesquiterpene synthases from grand fir (Abies grandis). Comparison of constitutive and wound-induced activities, and cDNA isolation, characterization, and bacterial expression of δ-selinene synthase and γ-humulene synthase
40. Testing the exon theory of genes: The evidence from protein structure
41. Maximizing the expression of mammalian cytochrome P-450 monooxygenase activities in yeast cells
42. Agrobacterium tumefaciens-mediated transformation of Arabidopsis root explants using kanamycin selection
43. Regulation and enzymology of pentacyclic triterpenoid phytoalexin biosynthesis in cell suspension cultures of Tabernaemontana divaricata