Cell identification and isolation on the basis of cytokine secretion: A novel tool for investigating immune responses

Nature Medicine

Volumn 7, Issue 3, 2001, Pages 373-376

  Turcanu, Victor ^a,b   Williams, Neil A ^a  

^a UNIVERSITY OF BRISTOL   (United Kingdom)

^b Sir Alexander Fleming Bldg   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

AVIDIN; GAMMA INTERFERON; INTERLEUKIN 10; INTERLEUKIN 4; TRANSFORMING GROWTH FACTOR BETA;

ARTICLE; CELL ISOLATION; CELL SELECTION; CELL TYPE; CONTROLLED STUDY; CYTOKINE RELEASE; HUMAN; HUMAN CELL; IMMUNE RESPONSE; MICROENCAPSULATION; PHENOTYPE; PRIORITY JOURNAL; T LYMPHOCYTE SUBPOPULATION;

CYTOKINES; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; IMMUNITY, CELLULAR;

EID: 0035106917     PISSN: 10788956     EISSN: None     Source Type: Journal    
DOI: 10.1038/85533     Document Type: Article

References (18)

1. Two types of mouse helper T-cell clone-implications for immune regulation
2. Peripheral deletion of antigen-reactive T cells in oral tolerance
3. + T-cell subset inhibits antigen-specific T-cell responses and prevents colitis
4. Gel microdroplets and flow cytometry: Rapid determination of antibody secretion by individual cells within a cell population
5. Gel microdrop technology for rapid isolation of rare and high producer cells
6. Secretion capture and report web: Use of affinity derivatized agarose microdroplets for the selection of hybridoma cells
7. High-sensitivity immunofluorescence for detection of the pro- and anti-inflammatory cytokines gamma interferon and interleukin-10 on the surface of cytokine-secreting cells
8. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies
9. Analysis and sorting of live cells according to secreted molecules, relocated to a cell-surface affinity matrix
10. Enrichment and detection of live antigen-specific CD4(+) and CD8(+) T cells based on cytokine secretion
11. Overexpression of autocrine TGFβ1 suppresses the growth of spindle epithelial cells in vitro and in vivo in the rat 4NQO model of oral carcinogenesis
12. Deposition of transforming growth factor-β in the marrow in myelofibrosis and the intracellular localization and secretion of TGF-β by leukemic cells
13. + T cells
14. Regulatory T cells and inflammatory bowel disease
15. Simultaneous quantitative determination of electroporative molecular uptake and subsequent cell survival using gel microdrops and flow cytometry
16. Incorporation of cells into an ELISA system enhances antigen driven lymphokine detection
17. Topics in the methodology of substitution reactions with agarose