Computer-based methods for the mouse full-length cDNA encyclopedia: Real-time sequence clustering for construction of a nonredundant cDNA library

Genome Research

Volumn 11, Issue 2, 2001, Pages 281-289

  Konno, Hideaki ^a,c   Fukunishi, Yoshifumi ^b,c   Shibata, Kazuhiro ^b   Itoh, Masayoshi ^b   Carninci, Piero ^b   Sugahara, Yuichi ^b   Hayashizaki, Yoshihide ^a,b,c  

^a RIKEN YOKOHAMA INSTITUTE   (Japan)

^b RIKEN TSUKUBA INSTITUTE   (Japan)

^c JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA;

3' UNTRANSLATED REGION; 5' UNTRANSLATED REGION; ARTICLE; COMPUTER SYSTEM; CONTROLLED STUDY; DATA BASE; DNA LIBRARY; DNA MICROARRAY; DNA SEQUENCE; GENE CLUSTER; GENE CONSTRUCT; GENE INSERTION; GENETIC SELECTION; METHODOLOGY; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; UNINDEXED SEQUENCE;

5' UNTRANSLATED REGIONS; ANIMALS; CLONING, MOLECULAR; CLUSTER ANALYSIS; COMPUTER SIMULATION; COMPUTER SYSTEMS; GENE LIBRARY; GENETIC VECTORS; MICE; MOLECULAR SEQUENCE DATA; QUALITY CONTROL; SEQUENCE ANALYSIS, DNA; SEQUENCE TAGGED SITES;

RODENTIA;

EID: 0035104193     PISSN: 10889051     EISSN: None     Source Type: Journal    
DOI: 10.1101/gr.GR-1457R     Document Type: Article

References (32)

1. Complementary DNA sequencing: Expressed sequence tags and human genome project
2. Sequence identification of 2375 human brain genes
3. Initial assessment of human gene diversity and expression patterns based upon 83 million nucleotides of cDNA sequence
4. Frequent alternative splicing of human genes
5. ESTablishing a human transcript map
6. D2uster: A validated method for clustering EST and full-length cDNA sequences
7. High-efficiency full-length cDNA cloning
8. High-efficiency full-length cDNA cloning by biotinylated CAP trapper
9. High efficiency selection of full-length cDNA by improved biotinylated cap trapper
10. Thermostabilization and thermoactivation of thermolabile enzymes by trehalose and its application for the synthesis of full length cDNA
11. Normalization and subtraction of cap-trapper selected cDNAs to prepare full-length cDNA libraries for rapid discovery of new genes
12. Alternative poly(A) site selection in complex transcription units: Means to an end?
13. Alternative polyadenylation in human mRNAs: A large-scale analysis by EST clustering
14. Expressed sequence tags and chromosomal localization of cDNA clones from subtracted retinal pigment epithelium library
15. CAP3: A DNA sequence assembly program
16. Automated filtration-based high-throughout plasmid preparation system
17. Single pass sequencing and physical and genetic mapping of human brain cDNAs
18. A comprehensive approach to clustering to expressed human gene sequence: The sequence tag alignment and consensus knowledge base
19. Increased specificity of reverse transcription priming by trehalose and oligo-blockers allows high-efficiency window separation of mRNA display
20. A general method applicable to the search for similarities in the amino acid sequence of two proteins
21. Large-scale cDNA sequencing analysis of quantitative and qualitative aspects of gene expression
22. Improved tools for biological sequence comparison
23. Assessing protein coding region integrity in cDNA sequencing projects
24. Characterization of gene expression in mouse blastocyst using single-pass sequencing of 3995 clones
25. Transcriptional sequencing: A method for DNA sequencing using RNA polymerase
26. Pieces of the puzzle: Expressed sequence tags and the catalog of human genes
27. A gene map of the human genome
28. High-efficiency cloning of Arabidopsis full-length cDNA by biotinylated CAP trapper
29. Comparative evaluation of 5′-end-sequence quality of clones in CAP trapper and other full-length-cDNA libraries
30. TIGR Assembler: A new tool for assembling large shotgun sequencing projects
31. 3′-End cleavage and polyadenylation of mRNA precursors
32. The biochemistry of 3′-end cleavage and polyadenylation of messenger RNA precursors