Production of recombinant human creatine kinase (r-hCK) isozymes by tandem repeat expression of M and B genes and characterization of r-hCK-MB

Clinical Chemistry

Volumn 47, Issue 3, 2001, Pages 471-476

  Sunahara, Y ^a   Uchida, K ^a   Tanaka, T ^a   Matsukawa, H ^a   Inagaki, M ^a   Matuo, Y ^a  

^a Oriental Yeast Co Ltd   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; CREATINE KINASE MB; PLASMID VECTOR; RECOMBINANT ENZYME;

ACUTE HEART INFARCTION; ARTICLE; CARDIOVASCULAR DISEASE; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYME PURIFICATION; ESCHERICHIA COLI; HUMAN; HUMAN TISSUE; PROTEIN EXPRESSION;

ESCHERICHIA COLI;

EID: 0035103005     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1093/clinchem/47.3.471     Document Type: Article

References (8)

1. Standardization of creatine kinase-MB (CK-MB) mass assays: The use of recombinant CK-MB as a reference material
2. Isolation sequence analysis of a full-length cDNA for human M creatine kinase
3. Recombinant M-B- and MB-type isozymes of human phosphoglyceric acid mutase: Their large-scale production and preparation of polyclonal antibodies specific to M- and B-type isozymes
4. Human creatine kinase: Isolation and sequence analysis of cDNA clones for the B subunit, development of subunit specific probes and determination of gene copy number
5. Measurement of protein using bicinchoninic acid
6. Determination of creatine kinase isoenzyme MB activity in serum using immunological inhibition of creatine kinase M subunit activity
7. Creatine kinase MB isoforms for early diagnosis and monitoring of acute myocardial infarction
8. Characterization of human creatine kinase BB and MB isoforms by means of isoelectric focusing