A simple, highly sensitive and improved method for the measurement of bleomycin-detectable iron: The 'catalytic iron index' and its value in the assessment of iron status in haemochromatosis

Clinical Science

Volumn 100, Issue 3, 2001, Pages 239-247

  Burkitt, M J ^a   Milne, L ^a   Raafat, A ^a  

^a MOUNT VERNON HOSPITAL   (United Kingdom)

Author keywords

Bleomycin assay; Free radicals; Haemochromatosis; Hydroxyl radical; Iron

Indexed keywords

BLEOMYCIN; ETHIDIUM; FERRITIN; IRON; THIOBARBITURIC ACID; TRANSFERRIN;

ARTICLE; ASSAY; CATALYSIS; CONTROLLED STUDY; DNA DEGRADATION; FERRITIN BLOOD LEVEL; HEMOCHROMATOSIS; HUMAN; IRON BLOOD LEVEL; IRON STORAGE; MEASUREMENT; METHODOLOGY; PERFORMANCE; PRIORITY JOURNAL; REFERENCE VALUE; VALIDATION PROCESS;

ANTIMETABOLITES, ANTINEOPLASTIC; BLEOMYCIN; DNA DAMAGE; ETHIDIUM; FERRITINS; HEMOCHROMATOSIS; HUMANS; IRON; REFERENCE VALUES; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; THIOBARBITURATES;

EID: 0035100244     PISSN: 01435221     EISSN: None     Source Type: Journal    
DOI: 10.1042/CS20000237     Document Type: Article

References (38)

1. Superoxide-dependent formation of hydroxyl radicals in the presence of iron salts
2. Radical-promoting loosely-bound iron in biological fluids and the bleomycin assay
3. Measurement of iron and copper in biological systems: Bleomycin and copper-phenanthroline assays
4. Role of free radicals and catalytic metal ions in human disease: An overview
5. Serum bleomycin-detectable iron in patients with thalassemia major with normal range of serum iron
6. Ferrous ions detected in cerebrospinal fluid by using bleomycin and DNA damage
7. Bleomycin-detectable iron in the plasma of premature and full-term neonates
8. Transient iron-overload with bleomycin-detectable iron present during cardiopulmonary bypass surgery
9. Presence of iron catalytic for free radical reactions in patients undergoing chemotherapy: Implications for therapeutic management
10. Non-transferrin bound iron and neutropenia after cytotoxic chemotherapy
11. Increase in bleomycin-detectable iron in ischaemia/reperfusion injury to rat kidneys
12. Direct evidence for in vivo hydroxyl-radical generation in experimental iron overload: An ESR spin-trapping investigation
13. Iron supplementation generates hydroxyl radical in vivo
14. The effect of iron overload on rat plasma and liver antioxidant status in vivo
15. A clinical evaluation of serum ferritin as an index of iron stores
16. Screening for hemochromatosis: A cost-effectiveness study based on 12,258 patients
17. Haemochromatosis
18. Determination of iron status: A brief review of physiological effects of iron measures
19. Malondialdehyde formation from lipid peroxides in the thiobarbituric acid test: The role of lipid radicals, iron salts, and metal chelators
20. The action of hydrogen peroxide on the formation of thiobarbituric acid-reactive material from microsomes, liposomes or from DNA damaged by bleomycin or phenanthroline. Artefacts in the thiobarbituric acid test
21. 'Free iron' and lipid peroxidation in the plasma of patients with iron overload
22. Inhibition of DNA-ethidium bromide intercalation due to free radical attack upon DNA. I. Comparison of the effects of various radicals
23. Copper-catalyzed DNA damage by ascorbate and hydrogen peroxide: Kinetics and yield
24. Copper-DNA adducts
25. Calcium indicator dye Quin2 inhibits hydrogen peroxide-induced DNA strand break formation via chelation of iron
26. In the absence of catalytic metals ascorbate does not autoxidize at pH 7: Ascorbate as a test for catalytic metals
27. Determination of aldehydic lipid peroxidation products: Malondialdehyde and 4-hydroxynonenal
28. Malondialdehyde determination as index of lipid peroxidation
29. Enhancement of bleomycin-iron free radical damage to DNA by antioxidants and their inhibition of lipid peroxidation
30. Review article: The screening, diagnosis and optimal management of haemochromatosis
31. Liver iron stores in patients with non-insulin-dependent diabetes mellitus
32. Body iron stores are associated with serum insulin and blood glucose concentrations. Population study of 1,013 eastern Finnish men
33. High stored iron levels are associated with excess risk of myocardial infarction in eastern Finnish men
34. Stored iron levels and myocardial infarction at young age
35. Iron stores are not associated with acute myocardial infarction
36. Body iron stores and the risk of coronary heart disease
37. No association between serum ferritin and asymptomatic carotis atherosclerosis
38. Iron and heart disease: Dangers of testing the wrong hypothesis