Comparison of dig-labeled PCR, nested PCR, and ELISA for the detection of Clavibacter michiganensis subsp. sepedonicus in field-grown potatoes

Plant Disease

Volumn 85, Issue 3, 2001, Pages 261-266

  Lee, I M ^a   Lukaesko, L A ^a   Maroon, C J M ^b  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

^b Agdia Inc   (United States)

Author keywords

Clavibacter michiganensis subsp; Insidiosus; Threshold values

Indexed keywords

ALKALINE PHOSPHATASE; BACTERIAL DNA; BACTERIUM DETECTION; DIGOXIGENIN; DNA SEQUENCE; ENZYME LINKED IMMUNOSORBENT ASSAY; MICROBIAL SENSITIVITY TEST; POLYMERASE CHAIN REACTION; POTATO; SEED; SIGNAL TRANSDUCTION; STEM; TRANSPOSABLE ELEMENT;

BACTERIA (MICROORGANISMS); CLAVIBACTER; CLAVIBACTER MICHIGANENSIS; CLAVIBACTER MICHIGANENSIS SUBSP. SEPEDONICUS; SOLANUM TUBEROSUM;

EID: 0035097475     PISSN: 01912917     EISSN: None     Source Type: Journal    
DOI: 10.1094/PDIS.2001.85.3.261     Document Type: Article

References (36)

1. Serological detection of nonmucoid strains of Clavibacter michiganensis subsp. sepedonicus in potato
2. Effect of inoculum dose and preparation, strain variation, and plant growth conditions on the egg-plant bioassay for bacterial ring rot
3. Characterization of a plasmid in isolates of Corynebacterium sepedonicum
4. Non-pathogenic bacteria associated with potato sterns cross-react with Corynebacterium sepedonicum antisera in immunofluorescence
5. Cross-reaction of Corynebacterium sepedonicum antisera with C. insidiosum, C. michiganese, and an unidentified coryneform bacterium
6. Detection of Clavibacter michiganensis subsp. sepedonicus in potato stems and tubers grown from seed pieces with various levels of inoculum
7. Determination of population densities of Corynebacterium sepedonicum in potato stem during the growing season
8. Evaluation of serological tests for detection of Clavibacter michiganenis subsp. sepedonicus in composite potato stem and tuber samples
9. Production of monoclonal antibodies to Corynebacterium sepedonicum
10. An ELISA test for bacterial ring rot of potato with a new monoclonal antibody
11. A plant DNA minipreparation: Version II
12. Specific detection of Clavibacter michiganenis subsp. sepedonicus with the polymerase chain reaction
13. Cloned diagnostic probe for the detection of Clavibacter michiganesis subsp. sepedonicus
14. Validating immunoassay test performance in the detection of Corynebacterium sepedonicum during the growing season
15. Specific identification of Clavibacter michiganesis subsp. sepedonicum by DNA-hybridization probes
16. Nested PCR assays for ultrasensitive detection of potato ring rot bacterium, Clavibacter michiganensis subsp. sepedonicus
17. An improved procedure for rapid and sensitive detection of the potato ring rot bacterium Clavibacter michiganensis subsp. sepedonicus in commercial potato tubers
18. Selection of polymerase chain reaction primers from an RNA intergenic spacer region for specific detection of Clavibacter michiganensis subsp. sepedonicus
19. Cross-reactions of Corynebacterium sepedonicum antisera with soil bacteria associated with potato tubers
20. Specific detection of Clavibacter michiganensis subsp. sepedonicus by amplification of three unique DNA sequences isolated by subtraction hybridization
21. Specific 16S ribosomal RNA targeted oligonucleotide probe against Clavibacter michiganensis subsp. sepedonicus
22. Distribution and partial characterization of pCS1, a highly conserved plasmid present in Clavibacter michiganense subsp. sependonicum
23. Genetic variation in strains of Clavibacter michiganense subsp. sepedonicum: Polymorphisms in restriction fragments containing a highly repeated sequence
24. Seed potato improvement in Canada
25. Corynebacterium sepedonicum in potato: Effect of inoculum concentration on ring rot symptoms and latent infection
26. Detection and identification of Clavibacter michiganensis subsp. sepedonicus and Clavibacter michiganensis subsp. michiganensis by nonradioactive hybridization, polymerase chain reaction, and restriction enzyme analysis
27. Inhibition of PCR components of food samples, microbial diagnostic assays and DNA-extraction solution
28. Detection of Clavibacter michiganensis subsp. sepedonicus in potato tubers by BIO-PCR and automated real- time fluorescence detection system
29. Detection of Clavibacter michiganensis subsp. sepedonicus by DNA amplification
30. Critical analyses of the principle of seed potato certification
31. Comparison of PCR, ELISA, and DNA hybridization for the detection of Clavibacter michiganensis subsp. sepedonicus in field-grown potatoes
32. Detection of low numbers of bacterial cells in soils and sediments by polymerase chain reaction
33. Synthetic and complex media for the rapid detection of fluorescence of phytopathogenic pseudomonads: Effect of the carbon source
34. Rapid and sensitive detection of Xanthomonas fragariae by simple alkaline DNA extraction and polymerase chain reaction