Use of short-lived green fluorescent protein for the detection of proteasome inhibition

BioTechniques

Volumn 30, Issue 3, 2001, Pages 656-660

  Andreatta, C ^a   Nahreini, P ^a   Hovland, A R ^a   Kumar, B ^a   Edwards Prasad, J ^a   Prasad, K N ^a  

^a UNIVERSITY OF COLORADO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BENZYLOXYCARBONYLLEUCYLLEUCYLLEUCINAL; COMPLEMENTARY DNA; GREEN FLUORESCENT PROTEIN; LACTACYSTIN; PROTEASOME; PROTEINASE INHIBITOR; VIRUS VECTOR;

ENZYME INHIBITION; EXPRESSION VECTOR; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMMUNOBLOTTING; NONHUMAN; PROTEIN EXPRESSION; RETROVIRUS; REVIEW;

MAMMALIA; UNIDENTIFIED RETROVIRUS;

EID: 0035096666     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/01303dd03     Document Type: Review

References (10)

1. Vesicular stomatitis virus G glycoprotein pseudotyped retroviral vectors: Concentration to very high titer and efficient gene transfer into mammalian and nonmammalian cells
2. Ubiquitin-mediated proteolysis: Biological regulation via destruction
3. Short-lived green fluorescent proteins for quantifying ubiquitin/proteasome-dependent proteolysis in living cells
4. Lactacystin, proteasome function, and cell fate
5. Green fluorescent protein (GFP): Applications in cell-based assays for drug discovery
6. Proteasome inhibitors: Valuable new tools for cell biologists
7. Generation of destabilized green fluorescent protein as a transcription reporter
8. Effects of altered cyclophilin A expression on growth and differentiation of human and mouse neuronal cells
9. Global unfolding of a substrate protein by the Hsp100 chaperone ClpA
10. Ubiquitination and deubiquitination: Targeting of proteins for degradation by the proteasome