PCR template preparation for capillary DNA sequencing

BioTechniques

Volumn 30, Issue 3, 2001, Pages 537-542

  Silva W A , Jr ^a   Costa, M C R ^a   Valente, V ^a   De Freitas Sousa, J ^a   Paco Larson M L ^a   Espreafico, E M ^a   Camargo, S S ^a   Monteiro, E ^a   De Jesus Holanda, A ^a   Zago, M A ^a   Simpson, A J G ^a   Neto, E D ^a  

^a LUDWIG INSTITUTE FOR CANCER RESEARCH   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

DNA;

ARTICLE; COST; DNA DETERMINATION; DNA SEQUENCE; DNA TEMPLATE; EXPRESSION VECTOR; HUMAN; OPEN READING FRAME; POLYMERASE CHAIN REACTION; QUALITY CONTROL; STANDARDIZATION;

BACTERIA (MICROORGANISMS);

EID: 0035095520     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/01303st05     Document Type: Article

References (12)

1. The genome sequence of Drosophila melanogoster
2. Brazilian scientists team up for cancer genome project
3. Shotgun sequencing of the human transcriptome with ORF expressed sequence tags
4. High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase
5. Base-calling of automated sequencer traces using Phred. 11. Error probabilities
6. Base-calling of automated sequencer traces using Phred I. Accuracy assessment
7. Simple and rapid preparation of plasmid template by a filtration method using microtiter filter plates
8. Turbo-Prep II: An inexpensive, high-throughput plasmid template preparation protocol
9. DNA sequencing with dye-labeled terminators and T7 DNA polymerase: Effect of dyes and dNTPs on incorporation of dye-terminators and probability analysis of termination fragments
10. A system for rapid DNA sequencing with fluorescent chain-terminating dideoxynucleotides
11. DNA sequencing with chain-terminating inhibitors
12. ONSA, the Sao Paulo Virtual Genomics Institute