Improved PCR-walking for large-scale isolation of plant T-DNA borders

BioTechniques

Volumn 30, Issue 3, 2001, Pages 496-504

  Balzergue, S ^a   Dubreucq, B ^a   Chauvin, S ^a   Le Clainche, I ^a   Le Boulaire, F ^a   De Rose, R ^a   Samson, F ^a   Biaudet, V ^a   Lecharny, A ^a   Cruaud, C ^a   Weissenbach, J ^a   Caboche, M ^a   Lepiniec, L ^a  

^a CEMAGREF   (France)

Author keywords

[No Author keywords available]

Indexed keywords

PLANT DNA;

ARABIDOPSIS; ARTICLE; DNA DETERMINATION; GENE AMPLIFICATION; GENETIC TRANSFORMATION; NONHUMAN; PLANT; POLYMERASE CHAIN REACTION;

ARABIDOPSIS; EMBRYOPHYTA;

EID: 0035095013     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/01303bm06     Document Type: Article

References (8)

1. In planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants
2. Efficient PCR walking on plant genomic DNA
3. Isolation of plant DNA from fresh tissue
4. General method for gene isolation in tagging approaches: Amplification of insertion mutagenised sites AIMS
5. Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR
6. Genetic applications of an inverse polymerase chain reaction
7. An improved PCR method for walking in uncloned genomic DNA
8. Screening of transgenic plants by amplification of unknown genomic DNA flanking T-DNA