Enhanced transformation efficiency of an anticoagulant hirudin gene into Saccharomyces cerevisiae by a Double δ-sequence

Journal of Microbiology and Biotechnology

Volumn 11, Issue 1, 2001, Pages 61-64

  Kim, M D ^a   Yoo, Y J ^a   Rhee, S K ^a   Seo, J H ^a  

^a Seoul National University   (South Korea)

Author keywords

intergration; Hirudin; Saccharomyces cerevisiae; Transformation efficiency

Indexed keywords

ANTICOAGULANT PROTEIN; DNA; HIRUDIN; SIGMA FACTOR;

ARTICLE; BACTERIAL GENE; CONCENTRATION RESPONSE; CONTROLLED STUDY; DNA INTEGRATION; DNA RECOMBINATION; EXPRESSION VECTOR; GENE CASSETTE; GENE EXPRESSION; GENE INSERTION; GENETIC RECOMBINATION; GENETIC TRANSFORMATION; MOLECULAR SIZE; NONHUMAN; SACCHAROMYCES CEREVISIAE; YEAST;

SACCHAROMYCES CEREVISIAE;

EID: 0035093694     PISSN: 10177825     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (20)

1. δ-Integration of endo/exo-glucanase and β-glucosidase genes into the yeast chromosomes for direct conversion of cellulose to ethanol
2. Novel SSF process for ethanol production from microcrystalline cellulose using the δ-integrated recombinant yeast, Saccharomyces cerevisiae L2612dGC
3. Effects of medium composition on hirudin production in recombinant Saccharomyces cerevisiae
4. Downstream processing of recombinant hirudin produced in Saccharomyces cerevisiae
5. Xylitol production using recombinant Saccharomyces cerevisiae containing multiple xylose reductase genes at chromosomal δ-sequences
6. Ty1-mediated integration of expression cassettes: Host strain effects, stability and product synthesis
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9. Pharmacological studies on the antithrombotic action of hirudin in experimental animal
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12. Production of hirudin by recombinant Saccharomyces cerevisiae in a membrane-recycle fermentor
13. Expression of Schwanniomyces occidentalis α-amylase gene in Saccharomyces cerevisiae var. diastaticus
14. Effect of galactose feeding on the improved production of hirudin in fed-batch cultures of recombinant Saccharomyces cerevisiae
15. Dithiothreitol improves the efficiency of yeast transformation
16. Foreign gene expression in yeast: A review
17. Gene expression and secretion of the anticoagulant hirudin in Saccharomyces cerevisiae
18. Heterologous gene expression and secretion of the anticoagulant hirudin in a methylotrophic yeast Hansenula polymorpha
19. The frequency of gene targeting in yeast depends on the number of target copies