Induction of senescence-associated genes by 5-bromodeoxyuridine in HeLa cells

Experimental Gerontology

Volumn 36, Issue 3, 2001, Pages 465-474

  Suzuki, T ^a   Minagawa, S ^a   Michishita, E ^a   Ogino, H ^a   Fujii, M ^a   Mitsui, Y ^b   Ayusawa, D ^a  

^a YOKOHAMA CITY UNIVERSITY   (Japan)

^b NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

Author keywords

5 Bromodeoxyuridine; cDNA subtraction; Gene expression; HeLa cell; Senescence

Indexed keywords

BROXURIDINE;

AGING; ARTICLE; CONTROLLED STUDY; DNA LIBRARY; GENE EXPRESSION; GENE EXPRESSION REGULATION; HELA CELL; HUMAN; HUMAN CELL; PRIORITY JOURNAL; SENESCENCE;

BROMODEOXYURIDINE; CELL AGING; COLLAGENASES; CYCLIN-DEPENDENT KINASE INHIBITOR P21; CYCLINS; EXPRESSED SEQUENCE TAGS; FIBRONECTINS; GENE EXPRESSION REGULATION; GENE LIBRARY; HELA CELLS; HUMANS; POLYMERASE CHAIN REACTION; TRANSCRIPTION, GENETIC;

MAMMALIA;

EID: 0035093310     PISSN: 05315565     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0531-5565(00)00223-0     Document Type: Article

References (38)

1. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose
2. Interaction of chromosomal proteins with BrdU substituted DNA as determined by chromatin-DNA competition
3. Inhibition of myoblast fusion after one round of DNA synthesis in 5-bromodeoxyuridine
4. On the mechanism of 5-bromodeoxyuridine induction of prolactin synthesis in rat pituitary tumor cells
5. 1-acetyltransferase
6. Isolation and characterization of a novel cDNA from HL-60 cells treated with 1,25-dihydroxyvitamin D-3
7. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease
8. Bromodeoxyuridine treatment of normal adult erythroid colonies: an in vitro model for reactivation of human fetal globin genes
9. Suppression subtractive hybridization: a versatile method for identifying differentially expressed genes
10. Induction of replicative senescence biomarkers by sublethal oxidative stresses in normal human fibroblast
11. Studies on the enhanced interaction of halodeoxyuridine-substituted DNAs with H1 histones and other polypeptides
12. 5-Bromodeoxyuridine specifically inhibits the synthesis of estrogen-induced proteins in MCF7 cells
13. Subtractive hybridization of mRNA from early passage and senescent endothelial cells
14. Cloning and identification of genes that associate with mammalian replicative senescence
15. The limited in vitro lifetime of human diploid cell strains
16. Induction of alkaline phosphatase by 5-bromodeoxyuridine in a hybrid line between mouse and Chinese hamster in culture
17. Fibronectin expression increases during in vitro cellular senescence: correlation with increased cell area
18. Enhanced expression of mitochondrial genes in senescent endothelial cells and fibroblasts
19. Histones bind more tightly to bromodeoxyuridine-substituted DNA than to normal DNA
20. The binding of lac repressor and the catabolite gene activator protein to halogen-substituted analogues of poly[d(A-T)
21. Cataloging altered gene expression in young and senescent cells using enhanced differential display
22. Mitotic misregulation and human aging
23. DNA topoisomerase inhibitors induce reversible senescence in normal human fibroblasts
24. 5-Bromodeoxyuridine induces senescence-like phenomena in mammalian cells regardless of cell type or species
25. Diverse gene sequences are overexpressed in Werner syndrome fibroblasts undergoing premature replicative senescence
26. Prohibitin, an evolutionarily conserved intracellular protein that blocks DNA synthesis in normal fibroblasts and HeLa cells
27. Human fibroblast commitment to a senescence-like state in response to histone deacetylase inhibitors is cell cycle dependent
28. Suppression of tyrosinase gene expression by bromodeoxyuridine in Syrian hamster melanoma cells is not due to its incorporation into upstream or coding sequences of the tyrosinase gene
29. Inhibition of myogenesis in a rat myoblast line by 5-bromodeoxyuridine
30. Cloning of cDNAs with possible association with senescence and immortalization of human cells
31. INK4a
32. Microarray analysis of replicative senescence
33. Control of interferon mRNA levels and interferon yields in butyrate and 5′-bromodeoxyuridine-treated Namalwa cells
34. cDNA sequence coding for a rat glia-derived nexin and its homology to members of the serpin superfamily
35. 5-Bromo-2′-deoxyuridine blocks myogenesis by extinguishing expression of MyoD1
36. Role of ceramide in cellular senescence
37. Mild hyperoxia shortens telomeres and inhibits proliferation of fibroblasts: a model for senescence?
38. Replicative senescence of human skin fibroblasts correlates with a loss of regulation and overexpression of collagenase activity