A comparison of F-actin labeling methods for light microscopy in different plant specimens: Multiple techniques supplement each other

Micron

Volumn 32, Issue 6, 2001, Pages 571-578

  Van Gestel, K ^a   Le, J ^a   Verbelen, J P ^a  

^a UNIVERSITY OF ANTWERP   (Belgium)

Author keywords

Confocal laser scanning microscopy; Glycerol; Microinjection; Phalloidin; Plant actin

Indexed keywords

ACTIN; DETERGENT; GLYCEROL; PHALLOIDIN;

ARABIDOPSIS; ARTICLE; CELL CULTURE; CHEMISTRY; COMPARATIVE STUDY; CONFOCAL MICROSCOPY; EVALUATION; IMMUNOHISTOCHEMISTRY; MAIZE; METHODOLOGY; MICROFILAMENT; PLANT; PLANT ROOT; PLANT SEED; TOBACCO; ULTRASTRUCTURE;

ACTINS; ARABIDOPSIS; CELLS, CULTURED; DETERGENTS; GLYCEROL; IMMUNOHISTOCHEMISTRY; MICROFILAMENTS; MICROSCOPY, CONFOCAL; PHALLOIDINE; PLANT ROOTS; PLANTS; PLANTS, TOXIC; SEEDS; TOBACCO; ZEA MAYS;

EID: 0035092945     PISSN: 09684328     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0968-4328(00)00054-8     Document Type: Article

References (32)

1. Characterization of a monoclonal antibody prepared against plant actin
2. Cryofixing single cells and multicellular specimens enhances structure and immunocytochemistry for light microscopy
3. The organization of the actin cytoskeleton in vertical and gravi-responding primary roots of maize
4. Stacks on tracks: The plant Golgi apparatus traffics on an actin/ER network
5. Redistribution of actin, profilin and phosphatidylinositol-4,5-bisphosphate in growing and maturing root hairs
6. Microtubule and F-actin dynamics at the division site in living Tradescantia stamen hair cells
7. Micro-injection of pollen specific actin-depolymerizing factor, ZmADF1, reorientates F-actin strands in Tradescantia stamen hair cells
8. Actin-organelle interaction: Association with chloroplast in Arabidopsis leaf mesophyll cells
9. The late pollen-specific actins in angiosperms
10. High efficiency transient and stable transformation by optimized DNA microinjection into Nicotiana tabacum protoplasts
11. A GFP-mouse talin fusion protein labels plant actin filaments in vivo and visualizes the actin cytoskeleton in growing pollen tubes
12. Immunogold labelling of actin on sections of freeze-substituted plant cells
13. Two monoclonal antibodies to actin: One muscle selective and one generally reactive
14. Isovariant dynamics expand and buffer the responses of complex systems: The diverse plant actin gene family
15. Monitoring of the course of fixation of plant cells
16. The role of actin in root hair morphogenesis: Studies with lipochito-oligosaccharide as a growth stimulator and cytochalasin as an actin perturbing drug
17. Stop-and-go movements of plant Golgi stacks are mediated by the acto-myosin system
18. Improved staining of F-actin and co-localization of mitochondria in plant cells
19. Actin network as a normal component of the cytoskeleton in many vascular plants
20. Protoplasts: Isolation, culture, plant regeneration
21. Covisualization in living onion cells of putative integrin, putative spectrin, actin, putative intermediate filaments, and other proteins at the cell membrane and in an endomembrane sheath
22. The endomembrane sheath: A key structure for understanding the plant cell?
23. Microinjected fluorescent phalloidin in vivo reveals the F-actin dynamics and assembly in higher plant mitotic cells
24. Cortical fine actin filaments in higher plant cells visualized by rhodamine-phalloidin after pretreatment with m-maleimidobenzoyl N-hydrosuccinimide ester
25. Actin localization and function in higher plants
26. Organized F-actin is essential for normal trichome morphogenesis in Arabidopsis
27. An actin network is present in the cytoplasma throughout the cell cycle of carrot cells and associates with the dividing nucleus
28. Probing the plant actin cytoskeleton during cytokinesis and interphase by profilin microinjection
29. Controlling cellular development in a single cell system of Nicotiana
30. Mobile arrays of vacuole ripples are common in plant cells
31. Immunofluorescence detection of F-actin on low melting point wax sections from plant tissues
32. Freeze-shattering: A simple and effective method for pemeabilizating higher plant cell walls