Chromatographic resolution and quantitative assay of CNS tissue sphingoids and sphingolipids

Journal of Lipid Research

Volumn 42, Issue 2, 2001, Pages 301-308

  Dasgupta, S ^a   Hogan, E L ^a  

^a MEDICAL UNIVERSITY OF SOUTH CAROLINA   (United States)

Author keywords

Ceramide; Chromatography; Ganglioside; Neutral glycosphingolipid; Phospholipid; Sphingomyelin

Indexed keywords

CERAMIDE; GALACTOSYLCERAMIDE; GLYCOSPHINGOLIPID; PHOSPHATIDYLCHOLINE; PSYCHOSINE; SILICIC ACID; SOLVENT; SPHINGANINE; SPHINGOLIPID; SPHINGOMYELIN; SPHINGOSINE;

ANIMAL TISSUE; ARTICLE; CATTLE; CENTRAL NERVOUS SYSTEM; GAS CHROMATOGRAPHY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; METABOLISM; NONHUMAN; PRIORITY JOURNAL; THIN LAYER CHROMATOGRAPHY;

ANIMALS; CATTLE; CENTRAL NERVOUS SYSTEM; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, THIN LAYER; SPHINGOLIPIDS;

EID: 0035091921     PISSN: 00222275     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (52)

1. Functions of sphingolipids and sphingolipid breakdown products in cellular regulation
2. The sphingomyelin cycle and second messenger function of ceramide
3. Signal transduction of stress via ceramide
4. Lysosphingolipids inhibit protein kinase C: Implication for sphingolipidosis
5. Bifunctional role of glycosphingolipids: Modulators for transmembrane signaling and mediators for cellular interactions
6. Binding of tetanus toxin to somatic neutral hybrid cells with varying ganglioside composition
7. Developmental changes in ganglioside composition and synthesis in embryonic rat brain
8. GD3 ganglioside is prevalent in fully differentiated neurons from rat retina
9. Successive isolation and separation of the major lipid fractions including gangliosides from single biological samples
10. Branched monosialo gangliosides of the lacto-series isolated from bovine erythrocytes: Characterization of a novel ganglioside, NeuGc-isoOctaosylceramide
11. Ganglio-N-tetraosyl ceramide (GA1) of bovine and human brain: Molecular characterization and presence in myelin
12. Ceramide composition of the psoriatic scale
13. A modified benzidine method for the chromatographic detection of sphingolipids and acid polysaccharides
14. Chemistry of mammalian glycolipids
15. Quantitative determination of the neutral glycosylceramides in human blood
16. Gas-liquid chromatographic assay of lipid-bound sialic acids: Measurement of gangliosides in brain of several species
17. Methylation analysis of neuraminic acids by gas chromatography-mass spectrometry
18. A new procedure for the isolation of brain gangliosides, and determination of their long chain base compositions
19. Methods for methanolysis of sphingolipids and direct determination of long-chain bases by gas chromatography
20. 3-Ketosphingolipids: Application to determination of sphingolipids which contain 4-sphingenine
21. Analysis of galactosphingosine (psychosine) in the brain
22. Rapid and sensitive determination of sphingosine base and sphingolipids with fluorescamine
23. Determination of long-chain base in glycosphingolipids with fluorescamine
24. Simplified fluorometric assay for sphingosine bases
25. Accumulation of galactosylsphingosine (psychosine) in the twitcher mouse: Determination by HPLC
26. Quantitative isolation of total glycosphingolipids from animal cells
27. Stage-specific expression of fuconeolacto- (Lewis X) and ganglio-series neutral glycosphingolipids during brain development: Characterization of Lewis X and related glycosphingolipids in bovine, human and rat brain
28. The use of Sep-Pak cartridge during the isolation of ganglioside
29. N-acetylglucosaminyltransferase regulates the expression of neolactoglycolipids including sulfoglucuronylglycolipids in the developing nervous system
30. The BCA protein determination system: An analysis of several buffers, incubation temperature and protein standards
31. Thin-layer chromatography of human platelet phospholipids with fatty acid analysis
32. Neutral monoglycosylceramides in rat brain: Occurrence, molecular expression and developmental variation
33. Immunochemical detection of glycosphingolipids on thin-layer chromatograms
34. Glucosylceramide in the nervous system - A mini-review
35. 2 terminal kinase and the transcription factor ATF2 in the insulin-producing cell line Rlm5F
36. Ceramide in apoptosis - Does it really matter?
37. Free sphingoid bases in normal murine tissues
38. Quantitative analysis of free sphingoid bases in brain and spinal cord tissues by high-performance liquid chromatography with a fluorescent detection
39. Modulation of protein kinase C and diverse cell functions by sphingosine: A pharmacologically interesting compound linking sphingolipids and signal tranduction
40. Signaling through the sphingomyelin pathway
41. Plasmalopsychosine, a novel plasmal (fatty aldehyde) conjugate of psychosine with cyclic acetal linkage: Isolation and characterization from human brain white matter
42. Novel modification of glycosphingolipids by long-chain cyclic acetals: Isolation and characterization of plasmalocerebroside from human brain
43. Model compounds for plasmalo glycolipids: Preparation of long chain cyclic acetals of methyl β-D-galactopyranoside and determination of their regio- and stereochemistry by proton NMR
44. 6-Acyl-galactosylceramides of pig brain: Structure and fatty acid composition
45. Molecular characterization of gangliotetraosylceramide (GA1) in normal human brain and its developmental changes
46. Chemical and immunological characterization of galactosyl β1-3-globoside in bovine, human and rat brain
47. 4Cer; a naturally occuring novel neutral glycosphingolipid in bovine brain
48. Gangliosides of human, cat, and rabbit spinal cord and cord myelin
49. Gangliosides: Structure, isolation and analysis
50. Structure and distribution of gangliosides
51. Simultaneous quantitative determination method for sphingolipid metabolites by liquid chromatography/ionspray ionization tandem mass spectrometry
52. A simple method for the isolation and purification of total lipides from animal tissues