Efficient co-transformation of Nicotiana tabacum by two independent T-DNAs, the effect of T-DNA size and implications for genetic separation

Transgenic Research

Volumn 10, Issue 2, 2001, Pages 143-155

  McCormac, A C ^a   Fowler, M R ^b   Chen, D F ^c   Elliott, M C ^b  

^a UNIVERSITY OF SOUTHAMPTON   (United Kingdom)

^b DE MONTFORT UNIVERSITY   (United Kingdom)

^c JEALOTT S HILL INTERNATIONAL RESEARCH CENTRE   (United Kingdom)

Author keywords

Agrobacterium tumefaciens; Co transformation; Independent T DNAs; Multi T DNA binary vectors; Segregation; Tobacco

Indexed keywords

AGROBACTERIUM TUMEFACIENS; AGROBACTERIUM; BACTERIAL CELL; DNA TRANSFER; GENE SEGREGATION; GENETIC LINKAGE; GENETIC SCREENING; GENETIC SEPARATION; GENETIC STRAIN; GENETIC TRANSFORMATION; GENOTYPE; PLANT MODEL; PLASMID VECTOR;

DNA, BACTERIAL; GENE TRANSFER TECHNIQUES; PLANTS, GENETICALLY MODIFIED; PLANTS, TOXIC; PLASMIDS; RHIZOBIUM; TOBACCO; TRANSFORMATION, GENETIC;

AGROBACTERIUM; AGROBACTERIUM TUMEFACIENS; BACTERIA (MICROORGANISMS); EMBRYOPHYTA; NICOTIANA TABACUM; TABACUM;

EID: 0035087373     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1008909203852     Document Type: Article

References (14)

1. Co-transformation with one Agrobacterium tumefaciens strain containing two binary plasmids as a method for producing marker-free transgenic plants
2. Two T-DNAs co-transformed into Brassica napus by a double Agrobacterium tumefaciens infection are mainly integrated at the same locus
3. Two unlinked T-DNAs can transform the same tobacco plant cell and segregate in the F1 generation
4. T-DNA integration patterns in co-transformed plant cells suggest that T-DNA repeats originate from co-integration of separate T-DNAs
5. Frequencies of simultaneous transformation with different T-DNAs and their relevance to the Agrobacterium plant-cell interaction
6. A binary plant vector strategy based on separation of vir- and T-region of Agrobacterium tumefaciens Ti-plasmid
7. A simple and general method for transferring genes into plants
8. Rapid assay of foreign gene-expression in leaf-disks transformed by Agrobacterium tumefaciens: Role of T-DNA borders in the transfer process
9. The hypervirulence of Agrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside of T-DNA
10. Vectors carrying two separate T-DNAs for co-transformation of higher plants mediated by Agrobacterium tumefaciens and segregation of transformants free from selection markers
11. pBECKS2000: A novel plasmid series for the facile creation of complex binary vectors, which incorporates 'clean-gene' facilities
12. Segregation of genes transferred to one plant cell from two separate Agrobacterium strains
13. A revised medium for rapid growth and bioassays with tobacco tissue cultures
14. High-frequency linkage of co-expressing T-DNA in transgenic Arabidopsis thaliana transformed by vacuum-infiltration of Agrobacterium tumefaciens