Overexpression of the Golgi-localized enzyme α-mannosidase IIx in Chinese hamster ovary cells results in the conversion of hexamannosyl-N-acetylchitobiose to tetramannosyl-N-acetylchitobiose in the N-glycan-processing pathway

European Journal of Biochemistry

Volumn 268, Issue 5, 2001, Pages 1280-1288

  Oh eda, Masayoshi ^a   Nakagawa, Hiroaki ^a   Akama, Tomoya O ^a   Lowitz, Kevin ^a   Misago, Masahiro ^b   Moremen, Kelley W ^c   Fukuda, Michiko N ^a  

^a BURNHAM INSTITUTE   (United States)

^b UNIVERSITY OF OCCUPATIONAL AND ENVIRONMENTAL HEALTH   (Japan)

^c UNIVERSITY OF GEORGIA   (United States)

Author keywords

mannosidase II; mannosidase IIx; Golgi; HPLC; N glycan

Indexed keywords

4 UMBELLIFERYL ALPHA DEXTRO MANNOSIDE; ACETIC ACID DERIVATIVE; ALPHA MANNOSIDASE; ALPHA MANNOSIDASE IIX; CHITIN DERIVATIVE; HEXAMANNOSYL N ACETYLCHITOBIOSE; PROTEIN A; SWAINSONINE; TETRAMANNOSYL N ACETYLCHITOBIOSE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CHINESE HAMSTER; ENZYME ACTIVITY; GENE OVEREXPRESSION; GOLGI COMPLEX; HUMAN; HUMAN CELL; HYDROLYSIS; IMMUNOCYTOCHEMISTRY; NONHUMAN; OVARY CELL; PRIORITY JOURNAL; STRUCTURE ANALYSIS; SYNTHESIS;

ANIMALS; CARBOHYDRATE CONFORMATION; CARBOHYDRATE SEQUENCE; CHO CELLS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; COS CELLS; CRICETINAE; DISACCHARIDES; FLUORESCENT ANTIBODY TECHNIQUE; GOLGI APPARATUS; HELA CELLS; HUMANS; IMMUNOHISTOCHEMISTRY; MANNOSIDASES; MICE; POLYSACCHARIDES; RECOMBINANT FUSION PROTEINS; STAPHYLOCOCCAL PROTEIN A; SWAINSONINE; TRANSFECTION;

ANIMALIA; CRICETINAE; CRICETULUS GRISEUS;

EID: 0035082787     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2001.01992.x     Document Type: Article

References (30)

1. Assembly of asparagine-linked oligosaccharides
2. Biosynthetic controls that determine the branching and microheterogeneity of protein-bound oligosaccharides
3. Alpha-D-mannosidases of rat liver Golgi membranes. Mannosidase II is the GlcNAcMan5-cleaving enzyme in glycoprotein biosynthesis and mannosidases Ia and Ib are the enzymes converting Man9 precursors to Man5 intermediates
4. Congenital dyserythropoietic anaemia type II (HEMPAS) and its molecular basis
5. HEMPAS
6. Localization of the congenital dyserythroblastic anemia II locus to chromosome 20q11.2 by genomewide search
7. Genetic heterogeneity of congenital dyserythropoietic anemia type II
8. Exclusion of three candidate genes as determinants of congenital dyserythro-poietic anemia type II (CDA-II)
9. Alpha-mannosidase-II deficiency results in dysery-thropoiesis and unveils an alternate pathway in oligosaccharide biosynthesis
10. Purification and characterization of a novel broad specificity (α1-2, α1-3 and a1-6) mannosidase from rat liver
11. Molecular cloning and expression of cDNAs encoding human α-mannosidase II and a novel α-mannosidase IIx isozyme
12. Structure and transcriptional regulation of human alpha-mannosidase IIx (alpha-mannosidase II isotype) gene
13. Isolation, characterization, and expression of cDNAs encoding murine α-mannosidase II, a Golgi enzyme that controls conversion of high mannose to complex N-glycans
14. Carbohydrate structure of erythropoietin expressed in Chinese hamster ovary cells by a human erythropoietin cDNA
15. Biosynthesis and modification of Golgi mannosidase II in HeLa and 3T3 cells
16. The retention signal for soluble proteins of the endoplasmic reticulum
17. Molecular cloning and expression of cDNA encoding the enzyme that controls conversion of high-mannose to hybrid and complex N-glycans: UDP-N-acetylglucosamine: Alpha-3-D-mannoside beta-1,2-N-acetylglucos-aminyltransferase I
18. Endo-β-galactosidases
19. Identification of neutral and sialyl N-linked oligo-saccharide structures from human serum glycoproteins using three kinds of high-performance liquid chromatography
20. Golgi retention of a trans-Golgi membrane protein, galactosyltransferase, requires cysteine and histidine residues within the membrane-anchoring domain
21. Altered Golgi localization of core 2 b-1,6-N-acetylglucosamininyl transferase leads to decreased synthesis of branched O-glycans
22. Expression cloning of novel Galbeta (1-3/1-4) GlcNAc alpha 3-3 sialytransferase using lectin resistant selectin
23. Core fucosylation of high-mannose-type oligosaccharides in GlcNAc transferase-I deficient (Lec1) CHO cells
24. An alpha-mannosidase purified from Aspergillus saitoi is specific for alpha 1,2 linkages
25. Genetic control of yeast mannan structure. Biochemical basis for the transformation of Saccharomyces cerevisiae somatic antigen
26. Retention and retrieval in the endoplasmic reticulum and the Golgi apparatus
27. Kin recognition between medial Golgi enzymes in HeLa cells
28. Synthetic substrate analogues for UDP-GlcNAc: Man alpha 1-3 R beta 1-2-N-acetylglucosaminyl-transferase I. Substrate specificity and inhibitors for the enzyme
29. Asparagine-linked glycoprotein biosynthesis in rat epididymis. Presence of a mannosidase II-like enzyme
30. Subcellular distribution in rat liver of a novel broad specificity (α1-2, α1-3 and α1-6) mannosidase active on oligomannose glycans