Effect of substrate size on immunoinhibition of amylase activity

Journal of Clinical Laboratory Analysis

Volumn 15, Issue 2, 2001, Pages 64-70

  Warshawsky, Ilka ^a   Hortin, Glen L ^a  

^a NATIONAL INSTITUTES OF HEALTH   (United States)

Author keywords

Amylase; Amylase isoenzymes; Chromogenic substrates; Enzyme immunoassay; Immunoassay; Macromolecular substrates

Indexed keywords

AMYLASE; EPITOPE; GLUCOSE; MONOCLONAL ANTIBODY; POLYCLONAL ANTIBODY; STARCH;

ANTIBODY DETECTION; ARTICLE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME SUBSTRATE COMPLEX; GEL FILTRATION CHROMATOGRAPHY; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOREGULATION; INHIBITION KINETICS; PARTICLE SIZE;

AMYLASES; ANTIBODIES, BLOCKING; ANTIBODIES, MONOCLONAL; BINDING SITES, ANTIBODY; BINDING, COMPETITIVE; ENZYME ACTIVATION; ENZYME INHIBITORS; HUMANS; IMMUNE SERA; ISOENZYMES; MACROMOLECULAR SUBSTANCES; PANCREAS; PARTICLE SIZE; SALIVARY GLANDS; STARCH; SUBSTRATE SPECIFICITY;

EID: 0035073854     PISSN: 08878013     EISSN: None     Source Type: Journal    
DOI: 10.1002/jcla.3     Document Type: Article

References (23)

1. "Homogeneous" enzyme immunoassay: New immunochemical technique
2. Antibodies to biologically active molecules
3. Homogenous enzyme immunoassay for proteins employing β-galactosidase
4. Action of β-galactosidase on novel synthetic macromolecular substrates. A processive enzymic reaction controlled by coulombic interactions
5. Mechanism by which antibodies inhibit hapten-malate dehydrogenase conjugates
6. Evaluation of enzyme-multiplied immunoassay technique (EMIT) for determination of serum digoxin
7. Creatine kinase-MB immunoinhibition in the diagnosis of suspected acute myocardial infarction
8. A monoclonal antibody that specifically inhibits human salivary α-amylase
9. Pancreatic x-amylase assay employing the synergism of two monoclonal antibodies
10. Specific immunoassay of αamylase isoenzyme in human serum
11. Enzymes
12. Development of a direct assay for α-amylase
13. Amylase and lipase in the emergency department evaluation of acute pancreatitis
14. Biochemistry of human alpha amylase isoenzymes
15. Electrophoretic assays of amylase isoenzymes and isoforms
16. A new method for the determination of alpha-amylase
17. Corrected sequences of cDNAs for human salivary and pancreatic alpha-amylases
18. Macroamylases: Differences in activity against various-size substrates
19. Use of high-performance size-exclusion chromatography to measure protein molecular weight and hydrodynamic radius. An investigation of the properties of the TSK 3000 SW column
20. Introduction to biotechnical and biomedical applications of poly(ethylene glycol)
21. Hydrodynamic characterization of random coil polymers by size exclusion chromatography
22. Hydrolysis by human α-amylase of p-nitrophenyl oligosaccharides containing four to seven glucose units
23. Subsite mapping of the human pancreatic α-amylase active site through structural, kinetic, and mutagenesis techniques