β-Subunit 102-104 residues are crucial to confer FSH activity to equine LH/CG but are not sufficient to confer FSH activity to human CG

Journal of Endocrinology

Volumn 169, Issue 1, 2001, Pages 55-63

  Chopineau, M ^a   Martinat, N ^a   Galet, C ^a   Guillou, F ^a   Combarnous, Y ^a  

^a UMR85 PHYSIOLOGIE DE LA REPRODUCTION ET DES COMPORTEMENTS   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; AMINO ACID; ARGININE; CHORIONIC GONADOTROPIN; CHORIONIC GONADOTROPIN BETA SUBUNIT; COMPLEMENTARY DNA; DIMER; FOLLITROPIN; GLYCINE; LUTEINIZING HORMONE; LYSINE; PHENYLALANINE; PROLINE; VALINE;

AMINO ACID SUBSTITUTION; ARTICLE; BETA CHAIN; CONTROLLED STUDY; DNA TRANSFECTION; DONKEY; HORMONE ACTION; HORMONE BINDING; HORSE; HYBRID; NONHUMAN; PRIORITY JOURNAL; RECEPTOR BINDING; SITE DIRECTED MUTAGENESIS; SPECIES DIFFERENCE;

ANIMALS; BIOLOGICAL ASSAY; CHORIONIC GONADOTROPIN; COS CELLS; DNA PRIMERS; ENZYME-LINKED IMMUNOSORBENT ASSAY; EQUIDAE; FOLLICLE STIMULATING HORMONE; FOLLICLE STIMULATING HORMONE, BETA SUBUNIT; GLYCOPROTEIN HORMONES, ALPHA SUBUNIT; HUMANS; LEYDIG CELLS; LUTEINIZING HORMONE; MALE; MUTAGENESIS, SITE-DIRECTED; RATS; RECOMBINANT PROTEINS; SPECIES SPECIFICITY; TESTOSTERONE; TRANSFECTION;

EID: 0035072738     PISSN: 00220795     EISSN: None     Source Type: Journal    
DOI: 10.1677/joe.0.1690055     Document Type: Article

References (28)

1. Purification and characterization of donkey chorionic gonadotrophin
2. Hybrids from equine LH: Alpha enhances, beta diminishes activity
3. Cloning and analysis of the cDNA for the common α-subunit of the donkey pituitary glycoprotein hormones
4. Cloning and analysis of the cDNA encoding the horse and donkey luteinizing hormone β-subunits
5. Expression of horse and donkey LH in COS-7 cells: Evidence for low FSH activity in donkey LH as compared with horse LH
6. Evidence that the α-subunit influences the specificity of receptor binding of the equine gonadotrophins
7. Cloning, sequencing and functional expression of zebra (Equus burchelli) LH
8. Molecular basis of the specificity of binding of glycoprotein hormones to their receptors
9. Receptor binding and functional properties of chimeric human follitropin prepared by an exchange between a small hydrophylic intercysteine loop of human follitropin and human lutropin
10. Crystal structure of glycosylated fully active human follicle stimulating hormone
11. Expression of an in vitro biologically active equine LH/CG without C-terminal peptide (CTP) and/or β26-110 disulfide bridge
12. Rapid in vitro desensitization of the testosterone response in rat Leydig cells by sub-active concentration of porcine luteinizing hormone
13. hCGβ residues 94-96 alter LH activity without appearing to make key receptor contacts
14. Mutagenesis of the determinant loop region of human choriogonadotrophin β
15. Chicken lutropin acts like follitropin in rat ovarian follitropin receptor: An isoelectric focusing study
16. Crystal structure of human chorionic gonadotrophin
17. Enzyme immunoassay (EIA) for equine chorionic gonadotropin/pregnant mare serum gonadotropin (eCG/PMSG)
18. A new molecular variant of luteinizing hormone associated with female infertility
19. Characterization of the bioactivity of the mutation (Gly102 Ser) in human luteinizing hormone beta-subunit gene
20. The biological role of the carboxyl-terminal extension of human chorionic gonadotropin β-subunit
21. Co-evolution of ligand-receptor pairs
22. Glycoprotein hormones: Structure and function
23. Chemical, biological and immunological properties of pituitary gonadotrophins from the donkey (Equus asinus); comparison with the horse (Equus caballus)
24. A single gene encodes the β-subunits of the equine luteinizing hormone and chorionic gonadotropin
25. Influence of fetal genotype on the follicle-stimulating hormone/luteinizing hormone ratio of pregnant mare serum gonadotrophin
26. Nucleotide (cDNA) sequence encoding the horse gonadotrophin α-subunit
27. Structure of human chorionic gonadotrophin at 2.6 A resolution from MAD analysis of the selenomethionyl protein
28. Lysine residues 2 and 104 of the human chorionic gonadotrophin β subunit influence receptor binding