A microscopic and biochemical study of fragmentation phenotypes in stage-appropriate human embryos

Human Reproduction

Volumn 16, Issue 4, 2001, Pages 719-729

  Van Blerkom, Jonathan ^a,b   Davis, Patrick ^a,b   Alexander, Samuel ^b  

^a UNIVERSITY OF COLORADO SCHOOL OF MEDICINE   (United States)

^b ROSE MEDICAL CENTER   (United States)

Author keywords

'Comet' assay; Electron microscopy; Embryo fragmentation; Time lapse video microscopy; TUNEL

Indexed keywords

ADENOSINE TRIPHOSPHATE; DNA FRAGMENT; HYDROCORTISONE;

ARTICLE; BIOCHEMISTRY; BLASTOMERE; CELL STRUCTURE; COMET ASSAY; COMPETENCE; CONTROLLED STUDY; DNA BASE COMPOSITION; EMBRYO; EMBRYO RESORPTION; FERTILIZATION IN VITRO; GEL ELECTROPHORESIS; GENE STRUCTURE; HUMAN; LYSIS; MITOCHONDRION; NICK END LABELING; PHENOTYPE; RESTRICTION FRAGMENT; STAINING; TRANSMISSION ELECTRON MICROSCOPY;

EID: 0035067866     PISSN: 02681161     EISSN: None     Source Type: Journal    
DOI: 10.1093/humrep/16.4.719     Document Type: Article

References (28)

1. Human embryo fragmentation in vitro and its implications for pregnancy and implantation
2. Temporal and spatial aspects of fragmentation in early human embyros: Possible effects on developmental competence and association with the differential elimination of regulatory proteins from polarized domains
3. A cautionary note on the use of the TUNEL stain to determine apoptosis
4. Monoclonal antibody to single-stranded DNA is a specific and sensitive cellular marker of apoptosis
5. Prevention of twin pregnancy after in-vitro fertilization or intracytoplasmic sperm injection based on strict embryo criteria: A prospective randomized clinical trial
6. Embryo score to predict implantation after in-vitro fertilization: Based on 957 single embryo transfers
7. In-situ detection of fragmented DNA (TUNEL Assay) fails to discriminate among apoptosis, necrosis, and autolytic cell death: A cautionary note
8. Apoptosis in the human embryo
9. Evaluation of a new embryo-grading system to predict pregnancy rates following in vitro fertilization
10. Programmed cell death and human embryo fragmentation
11. Distinction of apoptotic and necrotic cell death in situ labelling of fragmented DNA
12. RNA synthesis and splicing interferes with DNA in situ labeling techniques used to detect apoptosis
13. Annexin V labelling and terminal transferase-mediated DNA end labelling (TUNEL) assay in human arrested embryos
14. Apoptosis, oncosis, and necrosis: An overview of cell death
15. Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: Inhibition by overexpression of Bcl-2 and Abl
16. Use of videocinematography to assess morphological qualities of conventionally cultured and cocultured embryos
17. Fragmentation and death (a.k.a. apoptosis) of ovulated oocytes
18. Microgel electrophoresis: Sensitivity, mechanisms, and DNA electrostretching
19. A simple technique for quantitation of low levels of DNA damage in individual cells
20. Assessment of DNA damage in individual hamster embryos by comet assay
21. Apoptosis in the degeneration process of unfertilized mouse ova
22. Development of human embryos to the hatched blastocyst stage in the presence of absence of a mololayer of Vero cells
23. Intrafollicular influences on human oocyte developmental competence: Perifollicular vascularity, oocyte metabolism, and mitochondrial function
24. DNA strand breaks and phosphatidylserine redistribution in newly ovulated and cultured mouse and human oocytes: Occurrence and relationship to apoptosis
25. Nuclear and cytoplasmic dynamics of sperm penetration, pronuclear formation and microtubule organization during fertilization and early preimplantation development in the human
26. Mitochondrial transfer between oocytes: Potential applications of mitochondrial donation and the issue of heteroplasmy
27. Differential mitochondrial inheritance between blastomeres in cleavage stage human embryos: Determination at the pronuclear stage and relationship to microtubular organization, ATP content and developmental competence
28. Detection of reactive oxygen species (ROS) and apoptosis in human fragment embryos