PCR-based RNA probes: A quick and sensitive method to improve whole mount embryo in situ hybridizations

BioTechniques

Volumn 30, Issue 4, 2001, Pages 769-774

  David, R ^a   Wedlich, D ^a  

^a UNIVERSITY OF ULM   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CLONING; ENZYMES; FLUORESCENCE; GENES; PROBES; REACTION KINETICS; RNA;

HYBRIDIZATION;

BIOTECHNOLOGY;

COMPLEMENTARY RNA; RNA;

ANALYTIC METHOD; ARTICLE; EMBRYO; FLUORESCENCE IN SITU HYBRIDIZATION; IMMUNOFLUORESCENCE; IN SITU HYBRIDIZATION; NONHUMAN; POLYMERASE CHAIN REACTION; RNA PROBE; RNA TRANSCRIPTION; XENOPUS;

AMPHIBIA; REPTILIA; XENOPUS;

EID: 0035054176     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/01304st02     Document Type: Article

References (9)

1. V(+)-fibronectin expression and localization prior to gastrulation in Xenopus laevis embryos
2. Xenopus cadherin-6 is expressed in he central and peripheral nervous system and in neurogenic placodes
3. Expression of Xenopus homologs of the beta-catenin binding protein pontin52
4. Xpitx-1: A homeobox gene expressed during pituitary and cement gland formation of Xenopus embryos
5. A Xenopus mRNA related to Drosophila twist is expressed in response to induction in the mesoderm and the neural crest
6. Conversion of Xenopus ectoderm into neurons by NeuroD, a basic helix-loop-helix protein
7. Synthesis of cRNA probes from PCR-generated DNA
8. Development of neurogenic placodes in Xenopus laevis
9. Expression of a Xenopus homolog of Brachyury (T) is an immediate-early response to mesoderm induction