In vitro metabolism of opioid tetrapeptide agonists in various tissues and subcellular fractions from rats

Peptides

Volumn 22, Issue 4, 2001, Pages 613-621

  Krondahl, E ^a   Von Euler Chelpin, H ^b   Orzechowski, A ^b   Ekstrom G ^b   Lennernas H ^a  

^a Department of Pharmacy   (Sweden)

^b ASTRAZENECA R AND D   (Sweden)

Author keywords

Blood; Deamidation; Homogenate; Metabolism; Opioid peptides; Peptide hydrolysis; Plasma; Subcellular fractions

Indexed keywords

MU OPIATE RECEPTOR AGONIST;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANIMAL TISSUE; ARTICLE; BLOOD; BRAIN HOMOGENATE; CARBOXY TERMINAL SEQUENCE; CELL FRACTIONATION; CELLULAR DISTRIBUTION; CONTROLLED STUDY; DEAMINATION; KIDNEY HOMOGENATE; LARGE INTESTINE; LUNG HOMOGENATE; LYSOSOME; MALE; MITOCHONDRION; NONHUMAN; PLASMA; PRIORITY JOURNAL; PROTEIN DEGRADATION; RAT; SMALL INTESTINE; SPLEEN; TISSUE HOMOGENATE;

EID: 0035047501     PISSN: 01969781     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0196-9781(01)00328-X     Document Type: Article

References (46)

1. Pulmonary delivery of peptide drugs: Effect of particle size on bioavailability of leuprolide acetate in healthy male volunteers
2. Distribution of brush-border membrane peptidases along the rat intestine
3. Stability of neurotensin and acetylneurotensin 8-13 in brush-border membrane, cytosol, and homogenate of rat small intestine
4. Soft drugs: Principles and methods for the design of safe drugs
5. Opiate receptor affinities and behavioral effects of enkephalin: Structure-activity relationship of ten synthetic peptide analogues
6. Comparison of buturylcholinesterase and acetylcholinesterase
7. 2,5] enkephalin in rats
8. A fluorimetric assay for measuring deamidase (lysosomal protective protein) using high-performance liquid chromatography
9. Metabolism of antisense oligonucleotides in rat liver homogenates
10. Enkephalin hydrolysis in homogenates of various absorptive mucosae of the albino rabbit: Similarities in rates and involvement of aminopeptidases
11. In vitro metabolism: Subcellular fractions
12. Human lysosomal protective protein has cathepsin A-like activity distinct from its protective function
13. Pulmonary metabolism of exogenous enkephalins in isolated perfused rat lungs
14. Pharmacological characterization of endomorphin-1 and endomorphin-2 in mouse brain
15. Amidase-like activity of calpain I and calpain II on substance P and its related peptides
16. Ectopeptidases
17. Peptides are better absorbed from the lung than the gut in the rat
18. Inhibition of leucine enkephalin metabolism in rat blood, plasma and tissues in vitro by an aminoboronic acid derivative
19. A peptidase in human platelets that deamidates tachykinins. Probable identity with the lysosomal "protective protein"
20. Inactivation of endothelin I by deamidase (lysosomal protective protein)
21. HIV proteinase as a target for drug action
22. 2
23. 2
24. The selective role of cathepsins B and D in the lysosomal degradation of endogenous and exogenous proteins
25. Rat jejunal permeability and metabolism of μ-selective tetrapeptides in gastrointestinal fluids from humans and rats
26. Investigations of the in vitro metabolism of three opioid tetrapeptides by pancreatic and intestinal enzymes
27. The challenge of proteolytic enzymes in intestinal peptide delivery
28. Design and characterization of orally active Arg-Gly-Asp peptidomimetic vitronectin receptor antagonist SB 265123 for prevention of bone loss in osteoporosis
29. Enzymatic barriers to peptide and protein absorption
30. Endogenous opioid peptides: Multiple agonists and receptors
31. Protein measurement with the folin phenol reagent
32. Preparation and characterization of subcellular fractions from the liver of C57B1/6 mice, with special emphasis on their suitability for use in studies of epoxide hydrolase activities
33. Structure-activity relationships of enkephalin-like peptides
34. Studies of the in vitro human plasma degradation of methionine-enkephalin
35. Improved duration of action of peptide drugs
36. Susceptibility of ebiratide to proteolysis in rat intestinal fluid and homogenates and its protection by various protease inhibitors
37. Mechanisms of macromolecule absorption by the lungs
38. Inhaled insulin
39. Liquid chromatographic study of the enzymatic degradation of endomorphins, with identification by electrospray ionization mass spectrometry
40. Sensitive method for measuring hydrolysis of enkephalins in plasma, using High Performance Liquid Chromatography with electrochemical detection
41. Further characterization of the in vitro hydrolysis of [Leu]- and [Met]enkephalin in rat plasma: HPLC-ECD measurement of substrate and metabolite concentrations
42. Enkephalin hydrolysis by mouse plasma in vitro
43. Cellular carboxypeptidases
44. Characterization of proteolytic activities of pulmonary alveolar epithelium
45. A potent and selective endogenous agonist for the μ-opiate receptor